摘要：本研究拟探讨尿中核苷在原发性肝癌诊断中的意义。尿中核苷在优化的250 mmol•L-1十二烷基磺酸钠（SDS）、20mmol•L-1四硼酸钠及40 mmol•L-1二水合磷酸二氢钠组成的缓冲液(pH=6.8)条件下，在未涂层石英毛细管上,10kV恒压，2psi×s进样量, 25℃下通过胶束电动力学毛细管色谱法（MECC）检测,检测波长为260nm。尿中核苷的提取通过苯基硼酸亲和凝胶法。通过上述方法，检测了20位健康人及20位原发性肝癌患者尿中核苷的含量。统计结果显示，原发性肝癌患者尿中核苷特别是修饰核苷的排放水平高于健康人。该方法为尿中核苷临床应用价值的探讨提供了一种手段。
Abstract: This study was designed to investigate the usefulness of urinary nucleosides in the diagnosis of primary hepatic carcinoma. By using micellar electrokinetic capillary chromatography (MECC )method , urinary nucleosides were determined in an uncoated 50cm×75μm i. d capillary tube using a optimized 250 mmol•L-1SDS–20 mmol•L-1 sodium tetraborate–40 mmol•L-1 sodium dihydrogenphosphate buffer（pH=6.8）, 10kV voltage, 2psi×s injection, 25℃ temperature and UV detection at 260nm. The nucleosides were extracted from urine by phenylboronate affinity gel chromatography. Ten kinds of normal and modified nucleosides were determined in urine samples from 20 healthy individuals and 20 patients of primary hepatic carcinoma, it was showed that the contents of urinary nucleosides excretion, especially modified nucleoside in patients of primary hepatic carcinoma were higher than those in normal healthy persons. These results provided a kind of probably means for the assessindividualst of the urinary nucleosides in cancer clinical diagnoses.
王宏光,邵寒娟,陈玉强,陈亮. 毛细管电泳法用于核苷分离的研究[J]. 中国生物工程杂志, 2006, 26(0): 37-40.
. Study of Nucleosides Separation by Capillary Electrophoresis. China Biotechnology, 2006, 26(0): 37-40.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/37
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