差异显示技术是通过比较不同类型细胞或同种细胞处于不同时期或阶段细胞中不同表达的基因。该方法简便、特异、敏感、可重复性强等特点，将成为分子生物学中显示细胞差异基因的良好方法。本文综述了差异显示技术的基本原理; 主要操作方法:mRNA抽提、逆转录、电泳显示差异、再扩增、验证和测序等。分析及其在肿瘤研究中的应用, 疾病相关基因探查, 细胞生长发育相关基因的显示, 不同因素诱导细胞相关基因的表达等方面现状。
Differential display is the available method used for comprehending the genic expression of different cells or the same cells in different periods or stages. Because of its simplicity, sensitivity, and reproducibility，it has become the most widely used technique for studying differential gene expression This paper summarized the principle of differential display technique and emphasized the relative techniques, which include the extraction and qualification of mRNA, RT, electrophoresis, reamplification，confirmation, sequencing and so on.At last, it introduced the status of this technique used in cancer research,detection of pathology related genes，display of cells growth related genes and the expression of cells gene induced by diferrent factors.
董加喜,陈郧东,李运南,马涧泉. 差异显示技术及其在显示相关基因中的应用现状[J]. 中国生物工程杂志, 2006, 26(0): 166-169.
. Differential display technique and its application in related genes display. China Biotechnology, 2006, 26(0): 166-169.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/166
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