探讨了超声辐照和振荡两种方式下对有机相中脂肪酶催化合成柠檬酸酯的影响。研究超声功率、有机溶剂、反应体系的水含量、pH等对超声辐照促进酯化反应的影响。超声场下脂肪酶的最适pH与振荡反应体系相比没有改变，而米氏常数Km值减小。研究表明适宜的超声辐照可显著提高有机相脂肪酶催化酯化反应的速度.
Comparative studies of enzymatic synthesis of citrate in the presence and absence of ultrasound were carried out in organic media. The influence of ultrasound power，organic solvent , water content，pH value on enzymatic synthesis was investigated. The optimal pH value for lipase subject to ultrasound was similar to that of the control condition，and Km value decreased. It was shown that suitable ultrasound could enhance reaction rate of lipase catalyzed－esterification in organic solvent.
刘勇,吾满江·艾力,张永学,夏木西卡玛尔. 超声辐照对有机相中脂肪酶活性的影响[J]. 中国生物工程杂志, 2006, 26(0): 132-135.
. Effect of Ultrasonic Irradiation on the Activity of Lipase in Organic Solvent. China Biotechnology, 2006, 26(0): 132-135.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/132
Cited
