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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2023, Vol. 43 Issue (12): 1-13    DOI: 10.13523/j.cb.2310065
综述     
窥探纳米世界:纳米流式检测技术的研发及单颗粒水平表征应用*
蔡年桂,陈欣,张清源,底浩楠,詹小贞,陈军岩,陈昊,颜晓梅()
厦门大学化学化工学院 谱学分析与仪器教育部重点实验室 福建省化学生物学重点实验室 厦门 361005
Exploring the Nanoworld: Development and Single-Particle-Level Characterization of Nano-flow Cytometry Technology
CAI Nian-gui,CHEN Xin,ZHANG Qing-yuan,DI Hao-nan,ZHAN Xiao-zhen,CHEN Jun-yan,CHEN Hao,YAN Xiao-mei()
Key Laboratory of Spectrochemical Analysis &Instrumentation, Ministry of Education, Fujian Provincial Key Laboratory of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
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摘要：

纳米颗粒的准确表征对于推动生命科学认知、改善疾病诊断和治疗以及促进纳米科技的发展至关重要。由于纳米颗粒存在高度的个体差异性和异质性,迫切需要开发单颗粒水平的快速检测技术。基于瑞利散射与鞘流单分子荧光检测技术研发的纳米流式检测技术(nano-flow cytometry,nFCM),能够以每分钟高达10 000颗粒的速率实现对人工合成纳米颗粒(7~500 nm)以及细胞外囊泡、病毒等天然生物纳米颗粒的粒径分布、颗粒浓度和生化性状的高灵敏、高选择性、高通量多参数分析。通过讨论nFCM研发的重要性、挑战、进展以及产业转化,回顾该技术在纳米颗粒研究中的应用,并展望其未来的应用前景。
关键词： 纳米流式检测技术;  单颗粒分析;  纳米颗粒;  病毒;  细胞外囊泡    
Abstract:

Accurate characterization of nanoparticles is of paramount importance for advancing our understanding of life sciences, improving disease diagnosis and treatment, and fostering the development of nanotechnology. Due to the high degree of individual variation and heterogeneity among nanoparticles, there is an urgent need for the development of rapid detection techniques at the single-particle level. This review article introduces the development of nano-flow cytometry (nFCM), a technology based on Rayleigh scattering and sheath-flow single-molecule fluorescence detection. nFCM can achieve highly sensitive, selective, and high-throughput multi-parameter analysis of the size distribution, particle concentration, and biochemical characteristics of synthetic nanoparticles (7 ~ 500 nm), as well as naturally occurring biological nanoparticles such as extracellular vesicles and viruses, at a rate of up to 10 000 particles per minute. The article discusses the significance, challenges, progress, and industrial transformation of the nFCM development, reviews its applications in nanoparticle research, and discusses its future application prospects.
Key words: Nano-flow cytometry    Single-particle analysis    Nanoparticles    Virus    Extracellular vesicles
收稿日期: 2023-10-09 出版日期: 2024-01-16
ZTFLH:  Q819  
基金资助: *国家自然科学基金(21627811);国家自然科学基金(21934004);国家重点研发计划(2021YFA0909400)
通讯作者: **电子信箱: xmyan@xmu.edu.cn   
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蔡年桂
陈欣
张清源
底浩楠
詹小贞
陈军岩
陈昊
颜晓梅

引用本文:

蔡年桂, 陈欣, 张清源, 底浩楠, 詹小贞, 陈军岩, 陈昊, 颜晓梅. 窥探纳米世界:纳米流式检测技术的研发及单颗粒水平表征应用*[J]. 中国生物工程杂志, 2023, 43(12): 1-13.

Nian-gui CAI, Xin CHEN, Qing-yuan ZHANG, Hao-nan DI, Xiao-zhen ZHAN, Jun-yan CHEN, Hao CHEN, Xiao-mei YAN. Exploring the Nanoworld: Development and Single-Particle-Level Characterization of Nano-flow Cytometry Technology. China Biotechnology, 2023, 43(12): 1-13.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2310065    或    https://manu60.magtech.com.cn/biotech/CN/Y2023/V43/I12/1

图1  nFCM的仪器示意图及应用领域
图2  nFCM在纳米材料单颗粒水平表征中的应用
图3  nFCM在病毒单颗粒水平表征中的应用
图4  nFCM在纳米药物单颗粒水表征中的应用
图5  nFCM在EVs单颗粒水平表征中的应用
图6  nFCM在工程化EVs单颗粒水平表征中的应用
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