细胞催化技术生产醇类物质的研究进展<sup>*</sup>

doi:10.13523/j.cb.2209048

中国生物工程杂志

2023, Vol. 43

Issue (4): 123-132 DOI: 10.13523/j.cb.2209048

综述

细胞催化技术生产醇类物质的研究进展^*

张琰,刘袖洞(

)

大连大学环境与化学工程学院大连 116622

Research Progress of Alcohol Production by Cell Catalytic Technology

ZHANG Yan,LIU Xiu-dong(

)

College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China

全文: PDF(481 KB) HTML

摘要：

全细胞催化作为生物催化的一个重要分支,以完整的细胞为催化剂,避免了细胞裂解和酶纯化步骤,极大地削减了生产成本。同时,细胞壁成分可以保护胞内酶不受外界环境的影响,能够满足对催化剂的低成本和高稳定性的要求。对适宜乙醇、二醇、糖醇等醇类物质生产的菌株以及目前食品、化工工业中利用细胞催化技术生产醇类物质的应用进行综述,探讨提高醇类物质产量的生产策略,以期为细胞催化转化生产醇类物质的研究与工业开发提供参考。

关键词： 生物催化; 细胞催化; 乙醇; 二醇; 糖醇

Abstract:

As an important part of biocatalysis, whole-cell catalysis uses whole cells as catalysts, which eliminates the need of cell lysis and enzyme purification in the process of alcohol production, and thus greatly reduces the cost of alcohol production. At the same time, the cell wall components can protect the trapped enzyme from potentially harmful environmental factors, thus meeting the production requirements of low cost and high stability of catalysts. According to the advantages of cell catalysis technology and the current market application prospects, the suitable strains for producing alcohol substances, such as ethanol, diol and sugar alcohol, were discussed. The alcohol production by using cell catalysis technology in food and chemical industries was reviewed. Finally, the production strategy of increasing alcohol yield was also discussed.

Key words: Biocatalysis Cell catalysis Ethanol Glycol Sugar alcohol

收稿日期: 2022-09-17 出版日期: 2023-05-04

ZTFLH:

Q819

基金资助: 辽宁省教育厅高等学校基本科研项目(LJKZ1183)

通讯作者: **电子信箱:liuxiudong@dlu.edu.cn

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引用本文:

张琰, 刘袖洞. 细胞催化技术生产醇类物质的研究进展^*[J]. 中国生物工程杂志, 2023, 43(4): 123-132.

ZHANG Yan, LIU Xiu-dong. Research Progress of Alcohol Production by Cell Catalytic Technology. China Biotechnology, 2023, 43(4): 123-132.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2209048 或 https://manu60.magtech.com.cn/biotech/CN/Y2023/V43/I4/123

产物	菌株	底物	产量/ (g·L^-1)	得率 (g/g)	生产规模	参考文献
乙醇	酿酒酵母(S. cerevisiae WXY12)	葡萄糖和木糖	46.87	0.15	摇瓶	[8]
	运动发酵单胞菌(DSM 473)	葡萄糖	47.93	0.475	摇瓶	[9]
	产气大肠杆菌(TISTR 1468)	甘油和乙酸	32.1	0.125	100 mL生物反应器	[10]
	酿酒酵母(S. cerevisiae)	玉米秸秆汁液	62.12	0.45	摇瓶	[11]
	酿酒酵母(S. cerevisiae)	龙舌兰 (Agave tequilana)叶(一种农业残留物)	12.2±0.3	0.41	摇瓶	[12]
	大肠杆菌(Escherichia coli SL100)	大麦秸秆	19.43	0.16	4.5 L生物反应器	[13]
	假丝酵母(JCM 1644)	木质纤维素	55.64	0.45	摇瓶	[14]
2-苯乙醇	酿酒酵母(S. cerevisiae YS58)	L-苯丙氨酸	6.3	0.7	5 L生物反应器	[15]
	酿酒酵母(S. cerevisiae YS58)	L-苯丙氨酸	3.73	0.68	摇瓶	[16]
	酿酒酵母(S. cerevisiae MT2)	L-苯丙氨酸	3.59	0.72	摇瓶	[17]
	大肠杆菌(E. coli BW25113)	L-苯丙氨酸	5.32	0.65	200 μL全细胞催化生物反应器	[18]
	地衣芽孢杆菌(Bacillus licheniformis DW2)	L-苯丙氨酸	5.16	0.65	摇瓶	[19]
	酿酒酵母(S. cerevisiae zzuli-1)	烟草废料提取物	1.65	0.34	摇瓶	[20]
	马克斯克鲁维酵母(Kluyveromyces marxianus)	甜乳清	1.2	0.39	摇瓶	[21]
1, 3- 丙二醇	肺炎克雷伯氏菌(Klebsiella pneumoniae)	葡萄糖和戊糖	20.88	0.69	摇瓶	[22]
	肺炎克雷伯氏菌(K. pneumoniae)	葡萄糖和粗甘油	29.69	0.84	5 L生物反应器	[23]
	丁酸梭菌(DSM 4278)	粗甘油	12.69± 0.9	0.35± 0.02	1.3 L生物反应器	[24]
	罗伊氏乳杆菌(Lactobacillus reuteri CH53)	葡萄糖和甘油	68.32± 0.84	0.83± 0.02	5 L生物反应器	[25]
	谷氨酸棒杆菌(Corynebacterium glutamicum)	葡萄糖和木糖	110.4	0.42	5 L生物反应器	[26]
	酿酒酵母(S. cerevisiae)	甘油	3.58	0.15	摇瓶	[27]
2, 3- 丁二醇	肺炎克雷伯氏菌(K. pneumoniae)	葡萄糖	91	0.45	摇瓶	[28]
	巴西类芽孢杆菌(Paenibacillus brasilensis PB24)	葡萄糖	27	0.43	摇瓶	[29]
	路德维希肠杆菌(Enterobacter ludwigii)	蔗糖和甘蔗糖蜜	50.6	0.31	6.7 L生物反应器	[30]
	地衣芽孢杆菌(B. licheniformis YNP5-TSU)	葡萄糖	99.3	0.41	1 L生物反应器	[31]
	酿酒酵母(S. cerevisiae BY4741)	红海藻(Gmandium amansii)水解产物	14.8	0.3	50 mL不锈钢间歇式反应器	[32]
	路德维希肠杆菌(E. ludwigii BW)	葡萄糖和富含葡萄糖的水解产物	144.5	0.47	2.5 L生物反应器	[33]

表1 微生物菌株生产醇类物质的概况

表2 微生物催化生产糖醇概况

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