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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2022, Vol. 42 Issue (7): 90-100    DOI: 10.13523/j.cb.2203072
综述     
蓝藻光驱固碳合成糖类物质的技术研究进展*
曾雪霞1,2,3,但玉1,2,3,毛绍名1,2,**(),孙佳慧3,4,5,栾国栋3,4,5,**(),吕雪峰3,4,5
1. 中南林业科技大学生命科学与技术学院 长沙 410004
2. 中南林业科技大学林业生物技术湖南省重点实验室 长沙 410004
3. 中国科学院青岛生物能源与过程研究所 中国科学院生物燃料重点实验室 青岛 266101
4. 山东能源研究院 青岛 266101
5. 青岛新能源山东省实验室 青岛 266101
Research Progress on the Cyanobacterial Photosynthetic Production of Sugars Utilizing Carbon Dioxide
Xue-xia ZENG1,2,3,Yu DAN1,2,3,Shao-ming MAO1,2,**(),Jia-hui SUN3,4,5,Guo-dong LUAN3,4,5,**(),Xue-feng LV3,4,5
1. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China
2. Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry & Technology, Changsha 410004, China
3. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China
4. Shandong Energy Institute, Qingdao 266101, China
5. Qingdao New Energy Shandong Laboratory, Qingdao 266101, China
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摘要：

糖类物质在食品、医药、日化、发酵领域有着广泛应用,对人类健康和社会发展有着重要意义。发展新型糖类物质合成技术有利于解决传统植物生物质“采集-炼制”产糖模式所面临的高成本、长周期、时空限制等风险和问题。蓝藻是一类重要的光自养微生物,也是极具潜力的新型微生物光合平台,发展蓝藻光驱固碳产糖技术有望实现二氧化碳向特定糖类产物的一站式定向转化,实现糖类物质合成的模式变革。糖类物质本身在蓝藻天然光合代谢网络中发挥重要作用,特别是卡尔文循环、糖原代谢、相容性物质代谢等几个重要生理模块的运转都是以不同糖类物质的转化来驱动的;而合成生物技术的发展又为光合产糖网络重塑和扩展注入了新的驱动力,在产品类型、合成模式及生产效率上显著提升了蓝藻光驱固碳产糖技术的发展和应用潜力。针对蓝藻光驱固碳产糖技术的发展应用,从模式、策略、产物等不同维度总结了相关进展和风险挑战,并对其未来前景和方向进行了展望。
关键词： 蓝藻;  光合作用;  糖;  合成生物学    
Abstract:

Sugar substances are widely used in the fields of food, medicine, daily chemical and fermentation, and are of great significance to human health and social development. For a long time, the production of sugar substances has been practically based on the collection and planting-harvesting of plant biomass. Solar energy and carbon dioxide would be fixed in plant photosynthesis process, and stored in the form of sugars (starch, cellulose, and sucrose) in biomass. The bulk sugar feedstock would be extracted from plant biomass, and then be utilized for generation of other sugar products through multiple steps of conversion, refinery, and purification. The dependence on the plant sourced biomass of current sugar production technology leads to the unavoidable risks and drawbacks of long cultivation term, climate & location constraints, and high pre-treatment costs. The development of novel technologies for sugar production is conducive to removing the bottlenecks faced by the traditional modes derived from plant biomass harvesting-refining industry. Cyanobacteria are an important group of photoautotrophic prokaryotic microorganisms and are also supposed to be promising microbial photosynthetic platforms. The development of cyanobacterial synthetic biotechnology has facilitated the direct conversion of carbon dioxide into dozens of natural and non-natural metabolites, of which sugars are a representative group. Cyanobacterial photosynthetic production of sugars is expected to realize the one-step conversion of carbon dioxide into specific sugar products and to refresh the paradigm of current sugar production technology. Sugar metabolites play important roles in the natural photosynthetic metabolic network of cyanobacteria, especially that the operations of several important physiological modules such as the Calvin cycle, glycogen metabolism, and compatible substance metabolism, are mainly promoted by the conversion and metabolism of multiple sugars and sugar-derived metabolites. Although some progress has been made in the synthesis of special sugar substances using natural cyanobacterial resources, such as the successful development and industrial application of the technology of Spirulina large-cultivation based glycerol glucosides production, there are still many challenges in the development and application of this mode. In recent years, the development of synthetic biotechnology has been providing new driving forces for remodeling and expanding the cyanobacterial photosynthetic sugar metabolism networks. Through specific transporter engineering, secretory production of some important sugar products, e.g. sucrose and trehalose, has been achieved, and it significantly relieved the metabolic stress from intracellular storage and increased the product titers. In addition, the sugar secretions would facilitate the development of derived technologies such as in situ separation and extraction of products as well as construction of artificial photosynthesis driven consortium. In combination with metabolic engineering strategies from multiple levels and steps, the production efficiency of cyanobacterial sugars would be significantly enhanced, photosynthetic production of novel sugar products would be achieved, and the updating of the sugar production routes could be expected. This review systematically summarized the progress and challenges in developing and utilizing cyanobacterial photosynthetic sugar production technologies, and discussed the future development prospects and research directions.
Key words: Cyanobacteria    Photosynthesis    Sugar    Synthetic biology
收稿日期: 2022-03-31 出版日期: 2022-08-03
ZTFLH:  Q493.4  
基金资助: *国家自然科学基金资助项目(31770092);国家自然科学基金资助项目(32070084)
通讯作者: 毛绍名,栾国栋     E-mail: msm526@163.com;luangd@qibebt.ac.cn
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曾雪霞
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引用本文:

曾雪霞,但玉,毛绍名,孙佳慧,栾国栋,吕雪峰. 蓝藻光驱固碳合成糖类物质的技术研究进展*[J]. 中国生物工程杂志, 2022, 42(7): 90-100.

Xue-xia ZENG,Yu DAN,Shao-ming MAO,Jia-hui SUN,Guo-dong LUAN,Xue-feng LV. Research Progress on the Cyanobacterial Photosynthetic Production of Sugars Utilizing Carbon Dioxide. China Biotechnology, 2022, 42(7): 90-100.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2203072    或    https://manu60.magtech.com.cn/biotech/CN/Y2022/V42/I7/90

图1  糖类物质参与的蓝藻(聚球藻PCC 7942)天然光合代谢网络
宿主 产物 改造策略 产量 引文
聚球藻UTEX 2973 蔗糖 导入大肠杆菌CscB蔗糖转运蛋白 8 g/L [45]
聚球藻PCC 7942 海藻糖 过量表达鱼腥藻MTS-MTH途径;阻断蔗糖合成途径;导入非洲摇蚊TreT海藻糖转运蛋白 5.7 g/L(半连续批次培养) [46]
聚球藻PCC 7942 果糖、葡萄糖 过量表达蔗糖水解酶InvA;导入来自运动发酵单胞菌的己糖转运蛋白Glf 27 mg/L, 5.4 mg/L [50]
集胞藻PCC 6803 赤藓糖醇 导入Trichosporonoides megachiliensis赤藓糖-4-磷酸-磷酸酶和来自酿酒酵母的藓糖还原酶 256 mg/L [48]
聚球藻PCC 7002 甘露糖醇 导入大肠杆菌甘露糖醇-1-磷酸脱氢酶和艾美尔式菌甘露糖醇-1-磷酸酶;阻断糖原合成途径 1.1 g/L [49]
聚球藻PCC 7942 木糖醇 导入大肠杆菌来源的木糖转运蛋白XylE和白色念珠菌木糖还原酶XR 33 g/L(木糖为底物)
表1  人工构建蓝藻光驱固碳产糖细胞工厂
图2  合成生物技术驱动的蓝藻光驱固碳产糖模式发展
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