角蛋白载药纳米颗粒的制备及药物可控释放性能研究<sup>*</sup>

doi:10.13523/j.cb.2104002

中国生物工程杂志

2021, Vol. 41

Issue (8): 8-16 DOI: 10.13523/j.cb.2104002

研究报告

角蛋白载药纳米颗粒的制备及药物可控释放性能研究^*

李佳欣¹,张正¹,刘赫¹,杨青¹,吕成志^2,^**(

),杨君^1,^**(

)

1 大连理工大学生物工程学院大连 116024
2 大连市皮肤病医院大连 116021

Preparation and Drug Release Properties of Keratin-loaded Nanoparticles

LI Jia-xin¹,ZHANG Zheng¹,LIU He¹,YANG Qing¹,LV Cheng-zhi^2,^**(

),YANG Jun^1,^**(

)

1 School of Bioengineering, Dalian University of Technology, Dalian 116024,China
2 Dalian Dermatosis Hospital,Dalian 116021,China

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摘要：

目的:以角蛋白作为药物载体材料,制备智能响应性药物递送系统,研究其药物装载和释放性能。方法:利用去溶剂法制备角蛋白纳米颗粒(KNP),以罗丹明B(RB)和姜黄素(Cur)为亲水性和疏水性模式药物,制备载药KNP。利用钨灯丝扫描电镜(SEM)、动态光散射(DLS)、傅里叶变换红外光谱(FTIR)和药物体外释放实验等对KNP的尺寸、形貌、结构、载药和释药性能进行研究。结果:成功制备出粒径均一、约为300 nm 的KNP,能够装载亲水性和疏水性药物。载药颗粒在体外释放研究中表现出pH和氧化还原双重响应性。结论:利用去溶剂法,简便、安全地制备了分散性良好且具有pH和氧化还原双重响应性释放特性的角蛋白载药纳米颗粒,为角蛋白作为智能响应型药物递送载体的研究和应用提供了参考。

关键词： 角蛋白; 纳米颗粒; 药物载体; pH响应性; 氧化还原响应性

Abstract:

Objective: Keratin was used as the drug carrier material, and the hydrophilic drug rhodamine and hydrophobic drug curcumin were loaded to prepare the intelligent responsive drug delivery system, and the pH and redox responsiveness of drug release were studied. Methods: The exopolysaccharide of Lactobacillus plantarum was introduced into the reaction system of sodium selenite and ascorbic acid, and KNP was synthesized at room temperature. The size, morphology, structure and stability of KNP were studied by tungsten filament scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and drug release experiments in vitro. Results:KNP with uniform particle size of about 300 nm was successfully prepared, which can load hydrophilic and hydrophobic drugs. Keratin based nanoparticles exhibited pH and glutathione (GSH) dual responsive release characters. In addition, these drug-loaded particles showed potent prolongation duration, good stability, sustainable and controllable release than the original drug. Conclusion: KNP with high stability, good water dispersion and responsiveness can be prepared simply and safely, and the drug delivery system of keratin is an appropriate candidate drug carrier.

Key words: Keratin Nanoparticles Drug carrier pH-responsive Redox-responsive

收稿日期: 2021-04-30 出版日期: 2021-08-31

ZTFLH:

Q816

基金资助: * 国家重点研发项目(2016YFD0200502);国家自然科学基金(31772193);国家自然科学基金(318030076);中央高校基本科研业务费项目资助项目(DUT19TD38)

通讯作者: 吕成志,杨君 E-mail: dlpfb126.com;junyang@dlut.edu.cn

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引用本文:

李佳欣,张正,刘赫,杨青,吕成志,杨君. 角蛋白载药纳米颗粒的制备及药物可控释放性能研究^*[J]. 中国生物工程杂志, 2021, 41(8): 8-16.

LI Jia-xin,ZHANG Zheng,LIU He,YANG Qing,LV Cheng-zhi,YANG Jun. Preparation and Drug Release Properties of Keratin-loaded Nanoparticles. China Biotechnology, 2021, 41(8): 8-16.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2104002 或 https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I8/8

图1 单因素条件下KNP的粒径大小

图2 KNP钨灯丝扫描电镜下形态

表1 KNP载药效率和ζ电位

图3 载药纳米颗粒的红外光谱图

图4 载药纳米颗粒在pH为5和7时的释放曲线(25℃)

图5 载药纳米颗粒在室温(25℃)下的氧化还原响应性体外释放

图6 罗丹明B纳米颗粒在酵母细胞中的摄取

图7 姜黄素纳米颗粒在酵母细胞中的摄取

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