噬菌体重组酶系统在合成生物学中的应用<sup>*</sup>

doi:10.13523/j.cb.2103055

中国生物工程杂志

2021, Vol. 41

Issue (8): 90-102 DOI: 10.13523/j.cb.2103055

综述

噬菌体重组酶系统在合成生物学中的应用^*

郭曼曼^1,^2,³,田开仁^1,^2,³,乔建军^1,^2,³,李艳妮^1,^2,^**(

)

1 天津大学化工学院制药工程系天津 300072
2 天津大学系统生物工程教育部重点实验室天津 300072
3 天津化学化工协同创新中心合成生物学平台天津 300072

Application of Phage Recombinase Systems in Synthetic Biology

GUO Man-man^1,^2,³,TIAN Kai-ren^1,^2,³,QIAO Jian-jun^1,^2,³,LI Yan-ni^1,^2,^**(

)

1 Department of Pharmaceutical Engineering, College of Chemical Engineering, Tianjin University, Tianjin 300072, China
2 Key Laboratory of Systematic Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China
3 Key Laboratory of Systematic Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China

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摘要：

合成生物学技术采用工程化设计理念,对生物体进行有目标的设计、改造乃至重新合成,对重塑非自然功能的“人造生命”具有重要意义。噬菌体重组系统具有高效、精确和广谱适用性等特点,在基因工程、代谢工程以及生物治疗等合成生物学领域得到了广泛的应用。从基因电路、体内遗传改造和体外重组等方面全面阐述了噬菌体重组系统在合成生物学研究的现状及热点,对当前该系统的局限性进行了探讨,并就未来的研究和发展趋势进行了展望。

关键词： 合成生物学; 噬菌体重组酶; 基因电路; 遗传改造; 体外重组

Abstract:

Synthetic biology is an emerging filed that aims to de novo design and synthesis of biological functional modules, gene circuits or artificial life with the standardized bioparts based on engineering principles. Since its inception in 1998, phage recombinase-mediated site-specific recombination, also known as recombineering, has revolutionized synthetic biology. Due to high efficiency, high precision, and broad-spectrum applicability, phage recombinases have been developed into powerful synthetic biology tools such as gene editing, DNA assembly, and gene-directed insertion. Yet, some applications, such as gene circuits, biocomputing, and biotherapy, need controlled excision, and this requires the number of well-characterized integrases and RDFs. Here, the classification and applicability of phage recombinases and focused on the application of phage recombinase systems in gene circuits design and construction, in vivo genetic modification and in vitro recombination were briefly introduced. Besides, the challenges of phage recombinase as tools for genetic engineering and sophisticated gene expression regulation as well as prospects for phage recombinases were analyzed.

Key words: Synthetic biology Phage recombinase Gene circuit Genetic modification In vitro recombination

收稿日期: 2021-03-23 出版日期: 2021-08-31

ZTFLH:

Q814

基金资助: * 国家自然科学基金资助项目(31770076)

通讯作者: 李艳妮 E-mail: liyanni@tju.edu.cn

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引用本文:

郭曼曼,田开仁,乔建军,李艳妮. 噬菌体重组酶系统在合成生物学中的应用^*[J]. 中国生物工程杂志, 2021, 41(8): 90-102.

GUO Man-man,TIAN Kai-ren,QIAO Jian-jun,LI Yan-ni. Application of Phage Recombinase Systems in Synthetic Biology. China Biotechnology, 2021, 41(8): 90-102.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2103055 或 https://manu60.magtech.com.cn/biotech/CN/Y2021/V41/I8/90

表1 噬菌体重组酶的分类及适用宿主

表2 基于噬菌体重组系统的基因电路

图1 基于噬菌体重组酶的基因电路(根据参考文献[8,44,60]修改)

表3 常见的基因编辑工具

图2 噬菌体重组酶在体内遗传改造中的应用(根据参考文献[28,35,74]修改)

表4 噬菌体重组酶在体内遗传改造中的应用

表5 常用的体外重组技术

表6 噬菌体重组酶在体外重组中的应用

图3 噬菌体重组酶在体外重组中的应用(根据参考文献[97-98,100]修改)

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