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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2019, Vol. 39 Issue (9): 58-61    DOI: 10.13523/j.cb.20190908
研究报告     
CD177 +中性粒细胞在溃疡性结肠炎患者外周血中的表达及临床意义
张艳婷,郭玉峰,杨芝红,杨少奇()
宁夏医科大学总医院 银川 750001
The Expression and Clinical Significance of CD177 + Neutrophils in Patients with Ulcerative Colitis
ZHANG Yan-ting,GUO Yu-feng,YANG Zhi-hong,YANG Shao-qi()
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摘要：

目的: 通过对比CD177 +中性粒细胞在溃疡性结肠炎(UC)患者与正常对照者外周血中的表达差异,分析CD177 +中性粒细胞在溃疡性结肠炎发生发展中的临床意义。 方法: 收集30例UC患者及20例正常对照者外周血,采用流式细胞术检测中性粒细胞CD177 +中性粒细胞表达情况,对比两组CD177表达差异。 结果： UC患者外周血中CD177 +中性粒细胞表达明显高于正常对照组(P<0.01),中度活动UC外周血CD177 +中性粒细胞表达较轻度者明显增高(P<0.05),UC患者外周血CD177 +中性粒细胞%与Mayo评分呈显著正相关(r=0.384,P=0.036)。 结论： CD177 +中性粒细胞在UC患者外周血表达明显增高,且与疾病活动程度密切相关,能够反映UC患者临床疾病活动程度。

关键词： CD177 +中性粒细胞;  溃疡性结肠炎;  外周血    
Abstract:

Objective: To analyze the clinical significance of CD177 + neutrophils in ulcerative colitis (UC) by comparing the expression differences of CD177 + neutrophils in peripheral blood of UC patients with normal controls. Methods: Peripheral blood of 30 UC patients and 20 normal controls were collected, and the expression of neutrophils CD177 + was detected by flow cytometry, and compare the expression difference of CD177 in the two groups. Results: The expression of CD177 + neutrophils in peripheral blood of UC patients was significantly higher than the normal control group (P<0.01), the expression of CD177 + neutrophils in peripheral blood of UC patients was significantly higher than that of the mild UC patients (P<0.05), and the count of CD177 + neutrophils in peripheral blood of UC patients was correlated with Mayo score (r=0.384,P=0.036). Conclusion: The expression of CD177 + neutrophils in UC patients was significantly increased and closely related to the activity of the disease, which may reflect the clinical activity of UC.

Key words: CD177 +Neutrophils    Ulcerative colitis    Peripheral blood
收稿日期: 2019-08-30 出版日期: 2019-09-20
ZTFLH:  Q291  
通讯作者: 杨芝红,杨少奇     E-mail: shaoqiynh@163.com
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引用本文:

张艳婷,郭玉峰,杨芝红,杨少奇. CD177 +中性粒细胞在溃疡性结肠炎患者外周血中的表达及临床意义[J]. 中国生物工程杂志, 2019, 39(9): 58-61.

ZHANG Yan-ting,GUO Yu-feng,YANG Zhi-hong,YANG Shao-qi. The Expression and Clinical Significance of CD177 + Neutrophils in Patients with Ulcerative Colitis. China Biotechnology, 2019, 39(9): 58-61.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190908    或    https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I9/58

图1  流式细胞术检测CD177+中性粒细胞
分组 n CD177+中性粒细胞% T值 P值
UC组 30 58.45±20.9* 9.032 0.000
对照组 20 20.95±7.32
表1  UC患者及对照组外周血CD177+中性粒细胞表达情况
分组 n CD177+中性粒细胞% T值 P值
轻度UC 14 43.2±13.2* -5.11 0.000
中度UC 16 71.8±16.9
表2  不同严重程度UC患者外周血CD177+中性粒细胞表达情况
图2  UC患者外周血中CD177+中性粒细胞%与Mayo评分相关性
图3  UC患者外周血中CD177+中性粒细胞%与ESR相关性
图4  UC患者外周血中CD177+中性粒细胞%与CD8+T淋巴细胞相关性
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