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利用CRISPR/Cas9技术构建稳定表达人白蛋白基因的中国仓鼠卵巢细胞系 * |
周松涛1,陈蕴2,龚笑海2,金坚2**(),李华钟1**() |
1 江南大学生物工程学院 无锡 214122 2 江南大学药学院 无锡 214122 |
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Using CRISPR/Cas9 Technology to Construct Human Serum Albumin CHO Stable Expression Cell Line |
Song-tao ZHOU1,Yun CHEN2,Xiao-hai GONG2,Jian JIN2**(),Hua-zhong LI1**() |
1 School of Biotechnology, Jiangnan University, Wuxi 214122, China 2 School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China |
引用本文:
周松涛,陈蕴,龚笑海,金坚,李华钟. 利用CRISPR/Cas9技术构建稳定表达人白蛋白基因的中国仓鼠卵巢细胞系 *[J]. 中国生物工程杂志, 2019, 39(4): 52-59.
Song-tao ZHOU,Yun CHEN,Xiao-hai GONG,Jian JIN,Hua-zhong LI. Using CRISPR/Cas9 Technology to Construct Human Serum Albumin CHO Stable Expression Cell Line. China Biotechnology, 2019, 39(4): 52-59.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190407
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https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I4/52
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[1] |
Wurm F M . Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnology, 2004,22(11):1393-1398.
doi: 10.1038/nbt1026
pmid: 15529164
|
[2] |
Wurm F M, Hacker D . First CHO genome. Nature Biotechnology, 2011,29(8):718-720.
doi: 10.1038/nbt.1943
|
[3] |
Kim J, Kim Y G, Lee G . CHO cells in biotechnology for production of recombinant proteins: current state and further potential. Applied Microbiology & Biotechnology, 2012,93(3):917-930.
doi: 10.1007/s00253-011-3758-5
pmid: 22159888
|
[4] |
Fischer S, Handrick R, Otte K . The art of CHO cell engineering: A comprehensive retrospect and future perspectives. Biotechnology Advances, 2015,33(8):1878-1896.
doi: 10.1016/j.biotechadv.2015.10.015
pmid: 26523782
|
[5] |
Wilson C, Bellen H J, Gehring W J . Position effects on eukaryotic gene expression. Annual Review of Cell Biology, 1990,6(1):679-714.
doi: 10.1146/annurev.cb.06.110190.003335
pmid: 2275824
|
[6] |
Lee J S, Kallehauge T B, Pedersen L E , et al. Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway. Scientific Reports, 2015,5:8572.
doi: 10.1038/srep08572
pmid: 4339809
|
[7] |
Jakociunas T, Jensen M K, Keasling J D . CRISPR/Cas9 advances engineering of microbial cell factories. Metabolic Engingeering, 2016,34:44-59.
doi: 10.1016/j.ymben.2015.12.003
pmid: 26707540
|
[8] |
Lee J S, Grav L M, Pedersen L E , et al. Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment. Biotechnology Bioengineering, 2016,113(11):2518-2523.
doi: 10.1002/bit.26002
pmid: 27159230
|
[9] |
Kito M, Itami S, Fukano Y , et al. Construction of engineered CHO strains for high-level production of recombinant proteins. Applied Microbiology & Biotechnology, 2002,60(4):442-448.
doi: 10.1007/s00253-002-1134-1
pmid: 12466885
|
[10] |
Gao J, Cha S, Jonsson R , et al. Detection of anti-type 3 muscarinic acetylcholine receptor autoantibodies in the sera of Sjogren’s syndrome patients by use of a transfected cell line assay. Arthritis Rheum, 2004,50(8):2615-2621.
doi: 10.1002/art.20371
|
[11] |
Huang Y, Li Y, Wang Y G , et al. An efficient and targeted gene integration system for high-level antibody expression. Journal of Immunological Methods, 2007,322(1):28-39.
doi: 10.1016/j.jim.2007.01.022
pmid: 17350648
|
[12] |
Schwenk F, Baron U, Rajewsky K , et al. A cre-transgenic mouse strain for the ubiquitous deletion of loxp-flanked gene segments including deletion in germ cells. Nucleic Acid Research, 1995,23(24):5080-5081.
doi: 10.1093/nar/23.24.5080
|
[13] |
Yang-Nim P, Daniel M, Eisenberg E , et al. Application of the FLP/FRT system for conditional gene deletion in yeast Saccharomyces cerevisiae. Yeast, 2011,28(9):673-681.
doi: 10.1002/yea.1895
pmid: 3169912
|
[14] |
Cong L, Ran F A, Cox D , et al. Multiplex genome engineering using CRISPR/Cas systems. Science, 2013,339(6121):819-823.
doi: 10.1126/science.1231143
|
[15] |
Lee J S, Grav L M, Lewis N E , et al. CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives. Biotechnology Journal, 2015,10(7):979-994.
doi: 10.1002/biot.201500082
pmid: 26058577
|
[16] |
Mali P, Yang L, Esvelt K M , et al. RNA-guided human genome engineering via Cas9. Science, 2013,339(6121):823-826.
doi: 10.1126/science.1232033
pmid: 3712628
|
[17] |
Hockemeyed D, Soldner F, Beard C , et al. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nature Biotechnology, 2009,27(9):851-857.
doi: 10.1038/nbt.1562
pmid: 19680244
|
[18] |
Zhou S T, Ding XF, Yang L , et al. Discover stable expression hot spot in genome of Chinese Hasmter ovary cells using lentivirus based random integration method. [2018-11-14].
|
[19] |
Stemmer M, Thumger T, Del S , et al. CCTop: An intuitive, flexible and reliable CRISPR/Cas9 target prediction tool. PLoS One, 2015,10(4):e0124633.
doi: 10.1371/journal.pone.0124633
pmid: 4409221
|
[20] |
Takata Y, Kondo S, Goda N , et al. Comparison of efficiency between FLPe and Cre for recombinase-mediated cassette exchange in vitro and in adenovirus vector production. Genes to Cells, 2011,16(7):765-777.
doi: 10.1111/j.1365-2443.2011.01526.x
pmid: 21707874
|
[21] |
Xu D, Chen Y, Jin J . Effect of signal peptide on the expression and secretion of hepatocyte growth factor in CHO. Chinese Journal of Cell Biology, 2016,38(12):1-7.
|
[22] |
Gilbert L A, Horlbeck M A, Adamson B , et al. Genome-scale CRISPR-mediated control of gene repression and activation. Cell, 2014,159(3):647-661.
doi: 10.1016/j.cell.2014.09.029
pmid: 25307932
|
[23] |
Shalem O, Sanjana N E, Hartenian E , et al. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 2014,343(6166):84-87.
doi: 10.1126/science.1247005
|
[24] |
Wang T, Wei J J, Sabatini D M , et al. Genetic screens in human cells using the CRISPR-Cas9 system. Science, 2014,343(6166):80-84.
doi: 10.1126/science.1246981
|
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