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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2019, Vol. 39 Issue (1): 55-62    DOI: 10.13523/j.cb.20190107
技术与方法     
利用静电纺丝技术制备聚己内酯-胶原复合包载碱性成纤维细胞生长因子手术缝纫线及其缓释性能的研究 *
宋奕1,**,张翠云1,李奕1,张素素1,潘舜1,陶云云1,许璐摇1,何华成2,***(),吴疆1,***()
1 温州医科大学药学院 温州 325035
2 温州大学化学与材料工程学院 温州 325035
Preparation of a Novel Surgical Sewing Thread with Control Release of Basic Fibroblast Growth Factor Through Electrospinning Technology
Yi SONG1,**,Cui-yun ZHANG1,Yi LI1,Su-su ZHANG1,Shun PAN1,Yun-yun TAO1,Lu-yao XU1,Hua-cheng HE2,***(),Jiang WU1,***()
1 School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
2 College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, China
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摘要：

目的:制备具有碱性成纤维细胞生长因子缓释性能手术缝纫线的复合材料,并探究其机械性能和体外释药情况。方法:利用静电纺丝技术,通过研究复合材料中物质成分的配比,优化包载效率,并研究聚己内酯-胶原复合结构 (PCL-Col) 对可控释放 bFGF 的影响来制备具有生物活性与药物缓释性能的手术缝纫线;观察缝纫线微观结构,检测缝纫线机械性能和载药量。结果:通过扫描电镜结果证明载药的手术缝纫线具有完整的线状结构。进一步通过拉伸与拉伸循环实验证明缝纫线具有良好的机械性能,最后通过酶联免疫吸附实验 (enzyme linked immunosorbent assay,ELISA) 证明所制备的手术缝纫线具备一定的药物缓释性能。结论:通过静电纺丝装置,优化纺丝参数,成功制备了PCL-Col-bFGF的手术缝线复合材料。该缝纫线满足缝合所需要的机械性能同时具备一定的bFGF缓释性能。
关键词： 静电纺丝;  生长因子;  机械性能;  缓释性能    
Abstract:

Objective: Composite materials that surgical sewing thread with basic fibroblast growth factor slow release performance were prepared, and exploring its mechanical properties and in vitro release.Methods:By means of electrospinning technology, the efficiency of packaging was optimized by studying the ratio of material composition in composite materials. Furthermore, the influence of polycaprolactone-collagen composite structure (PCL-Col) on the controllable release of bFGF was studied, to prepare the surgical sewing thread with biological activity and drug sustained release performance. Finally, the microstructure of the sewing thread was observed, and the mechanical properties and the drug loading capacity were measured.Results:The results of scanning electron microscopy (SEM) showed that the surgical sewing thread had a complete linear structure. It is proved that the sewing thread has good mechanical properties by stretching and stretching cycle experiments. Finally, enzyme linked immune sorbent assay (enzyme linked immunosorbent assay,ELISA) proved that the surgical sewing thread had a certain of drug release properties.Conclusion:By using electrospinning device and optimizing spinning parameters, PCL-Col-bFGF surgical suture composite was successfully prepared. The sewing thread meets the mechanical properties requirement of suturing and has bFGF sustained release performance.
Key words: Electrospinning    Growth factor    Mechanical property    Slow release performance
收稿日期: 2018-06-23 出版日期: 2019-02-28
ZTFLH:  Q813  
基金资助: * 2018年浙江省大学生科技创新活动计划暨新苗人才计划大学生科技创新项目(2018R413019);国家级大学生创新创业训练计划项目资助项目(201810343001)
通讯作者: 宋奕,何华成,吴疆     E-mail: hehc@wzu.ed.cn;woody870402@hotmail.com
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宋奕
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引用本文:

宋奕,张翠云,李奕,张素素,潘舜,陶云云,许璐摇,何华成,吴疆. 利用静电纺丝技术制备聚己内酯-胶原复合包载碱性成纤维细胞生长因子手术缝纫线及其缓释性能的研究 *[J]. 中国生物工程杂志, 2019, 39(1): 55-62.

Yi SONG,Cui-yun ZHANG,Yi LI,Su-su ZHANG,Shun PAN,Yun-yun TAO,Lu-yao XU,Hua-cheng HE,Jiang WU. Preparation of a Novel Surgical Sewing Thread with Control Release of Basic Fibroblast Growth Factor Through Electrospinning Technology. China Biotechnology, 2019, 39(1): 55-62.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20190107    或    https://manu60.magtech.com.cn/biotech/CN/Y2019/V39/I1/55

图1  PCL-Col-bFGF纳米纤维的制备流程图
组别 1 2 3 4 5 6 7
聚己内酯(g) 0.45 0.45 0.45 0.68 0.68 0.68 0.91
六氟异丙醇(ml) 6.50 6.75 7.00 6.50 6.75 7.00 6.75
水(ml) 0.50 0.25 0 0.50 0.25 0 0.25
鼠尾胶原蛋白(mg) 226.3 226.3 226.3 226.3 226.3 226.3 226.3
bFGF(μl) 113 113 113 113 113 113 113
体系均匀程度 优 优 bFGF析出 优 优 bFGF析出 体系不均匀
机械性能 + + + ++ +++ +++ -
表1  各物质配比设计
静电纺丝参数 电压(kV) 距离(cm) 喷速(mm/h) 接受器转速
(r/min)		 时间(min) 均匀程度 机械性能
1 6 12 1.08 300 5 + +
2 6 12 2.16 300 5 ++ +
3 6 12 3.24 300 5 + ++
4 12 12 1.08 300 5 ++ ++
5 12 12 2.16 300 5 +++ +++
6 12 12 3.24 300 5 + +
7 18 12 1.08 300 5 ++ +
8 18 12 2.16 300 5 ++ ++
9 18 12 3.24 300 5 + +
表2  静电纺丝各参数大小
图2  包载bFGF微粒缝纫线的合成和表征
图3  载药手术缝纫线单次拉伸图
弹性模量 (杨氏拉伸应力
5%~10%)(MPa)		 最大拉伸应力(MPa) 拉伸应变(位移)
在断裂(标准)(%)
PCL 0.011 62±0.002 60 0.002 43±0.000 30 69.837 19±0.281 72
PCL+Col 0.016 20±0.000 67 0.002 83±0.000 34 66.326 75±1.854 87
PCL+Col+bFGF 0.015 71±0.000 33 0.002 31±0.000 25 69.839 13±2.025 80
表3  三组线弹性模量、最大拉伸应力、断裂时拉伸应变对比
图4  载药手术缝纫线循环拉伸图
第一次循环
(J/m3)		 第二次循环
(J/m3)		 第三次循环
(J/m3)
PCL 0.831 7 2.175 1 3.185 8
PCL+Col 0.741 2 2.094 7 2.999 3
PCL+Col+bFGF 0.857 8 2.212 1 2.999 6
表4  三组循环拉伸损耗量表
图5  亲水性水接触角检测
时间(h) 在纯水中释放量
(ng)		 在PBS溶液中释放量
(ng)
6 50.11 48.06
12 90.34 95.31
24 128.60 138.73
48 163.19 184.13
72 197.72 229.30
96 236.99 273.99
120 275.30 320.51
144 301.86 366.70
168 337.99 410.83
表5  手术缝纫线载药释放检测
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