杂交瘤细胞体外大规模培养研究进展

doi:10.13523/j.cb.20181010

中国生物工程杂志

2018, Vol. 38

Issue (10): 82-89 DOI: 10.13523/j.cb.20181010

综述

杂交瘤细胞体外大规模培养研究进展

孙静静,周伟伟,周雷鸣,赵巧辉(

),李桂林

郑州安图生物工程股份有限公司郑州 450016

Advance in Large-Scale Culture of Hybridoma Cells in Vitro

Jing-jing SUN,Wei-wei ZHOU,Lei-ming ZHOU,Qiao-hui ZHAO(

),Gui-lin LI

Zhengzhou Autobio Diagnostic Co,LTD,Zhengzhou 450016,China

全文: PDF(736 KB) HTML

摘要：

单克隆抗体在生物学和医学研究领域中显示了极大的应用价值,是免疫检验中的新型试剂,是生物治疗的导向武器。作为医学检验试剂,单克隆抗体可以充分发挥其优势,如特异性好,灵敏度高,更便于质量控制,利于标准化和规范化。传统的方法是利用小鼠腹水制备单克隆抗体,但是近几十年杂交瘤细胞体外大规模培养制备单克隆抗体技术也在不断发展。特别是单克隆抗体在疾病诊断和治疗方面的需求,更进一步促进了杂交瘤细胞体外培养生产技术的发展,体外培养杂交瘤细胞生产的单克隆抗体已应用到许多方面。由于杂交瘤细胞的半贴壁性质,无论是悬浮培养还是贴壁培养,均可进行杂交瘤细胞的体外大规模培养。针对应用于体外诊断试剂的杂交瘤细胞体外培养制备单克隆抗体进行综述,主要包括中空纤维细胞培养和生物反应器细胞培养方法,以及不同培养方法优化的进展。

关键词： 杂交瘤细胞; 单克隆抗体; 生物反应器; 中空纤维

Abstract:

Monoclonal antibodies have shown great value in the field of biology and medical research. They are new reagents in immunoassays and are the guided weapons for biological therapy. As a diagnostic reagent in vitro, monoclonal antibody can give full play to its advantages, such as good specificity, high sensitivity, and more convenient for quality control, which is conducive to standardization .Traditionally,mouse ascites were used to prepare monoclonal antibodies. Now monoclonal antibodies are also being developed in large-scale cultures of hybridoma cells in vitro. In particular, the needs of monoclonal antibodies in the diagnosis and treatment of diseases have further promoted the development of invitro culture and production techniques for hybridoma cells. Due to the semi-adherent nature of the hybridoma cells, both in suspension and adherent culture, large-scale invitro culture of hybridoma cells can be performed. This article reviews the invitro culture techniques of hybridoma cells, including hollow fiber cell culture system and bioreactor cell culture sysytem, as well as the optimization of different culture methods.

Key words: Hybridoma Monoclonal antibody Bioreactor Hollow fiber

收稿日期: 2018-04-28 出版日期: 2018-11-09

ZTFLH:

Q813

通讯作者: 赵巧辉 E-mail: zhaoqiaohui@autobio.com.cn

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引用本文:

孙静静,周伟伟,周雷鸣,赵巧辉,李桂林. 杂交瘤细胞体外大规模培养研究进展[J]. 中国生物工程杂志, 2018, 38(10): 82-89.

Jing-jing SUN,Wei-wei ZHOU,Lei-ming ZHOU,Qiao-hui ZHAO,Gui-lin LI. Advance in Large-Scale Culture of Hybridoma Cells in Vitro. China Biotechnology, 2018, 38(10): 82-89.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20181010 或 https://manu60.magtech.com.cn/biotech/CN/Y2018/V38/I10/82

图1 生物反应器制备单克隆抗体上游成本

图2 中空纤维系统制备单克隆抗体上游成本

表1 生物反应器和中空纤维系统比较

图3 中空纤维细胞培养横截面[8]

表2 常见的培养过程参数变化产生影响

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