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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2017, Vol. 37 Issue (4): 40-47    DOI: 10.13523/j.cb.20170406
研究报告     
ANKRD49通过上调Bcl-xL的表达抑制UV诱导GC-1细胞的凋亡
白欣艳1, 温丽敏1, 王玉晶1, 王海龙2, 解军1, 郭睿1
1 山西医科大学生物化学与分子生物学教研室 太原 030001;
2 山西医科大学寄生虫学教研室 太原 030001
ANKRD49 Inhibits UV-induced Apoptosis of GC-1 Cells by Up-regulating Bcl-xL
BAI Xin-yan1, WEN Li-min1, WANG Yu-jing1, WANG Hai-long2, XIE Jun1, GUO Rui1
1 Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China;
2 Department of Parasitology, Shanxi Medical University, Taiyuan 030001, China
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摘要: 目的:利用稳定过表达Ankyrin repeat domain 49(ANKRD49)的GC-1(小鼠精原细胞)细胞模型,探讨ANKRD49过表达对GC-1细胞凋亡的影响。方法:40 J/m2 紫外线(UV)刺激GC-1细胞2 min 诱导凋亡,分别利用流式细胞术、Hoechst33258染色和免疫印迹技术检测过表达ANKRD49对GC-1细胞线粒体膜电位、核浓缩情况和凋亡相关蛋白多聚ADP核糖聚合酶(poly ADP-ribose polymerase,PARP)、Cleaved-Caspase-3、Bcl-xL和Bax的表达变化,以反映ANKRD49对GC-1细胞凋亡的影响。结果:流式细胞术检测结果表明稳定过表达ANKRD49的GC-1细胞线粒体膜电位下降率和细胞凋亡率均明显低于空载体组和裸细胞组(P<0.05)。Hoechst33258染色结果显示ANKRD49稳定过表达组细胞的凋亡百分比明显低于其它组(P<0.05)。Western blotting 检测结果显示ANKRD49稳定过表达组Cleaved-PARP、Cleaved-Caspase-3蛋白的表达水平明显低于对照组,而Bcl-xL蛋白表达水平明显高于对照组(P<0.05)。结论:ANKRD49过表达可抑制UV诱导的GC-1细胞凋亡,其作用可能是直接或间接通过促进Bcl-xL的表达而实现的。
关键词: GC-1细胞Bcl-xLANKRD49细胞凋亡    
Abstract: Objective:To study the effects of ANKRD49 on ultraviolet light (UV)-induced apoptosis of GC-1 cells. Methods:Apoptosis of GC-1 cells was induced by 40 J/m2 UV for 2 minutes. The effect of ANKRD49 on mitochondrial membrane potential and nuclear condensation of GC-1 cells was detected by flow cytometry and Hoechst33258 staining, respectively. The levels of PARP, Cleaved-Caspase-3, Bcl-xL and Bax were measured by Western blotting. Results:The results of flow cytometry showed that the rates of decline of mitochondrial membrane potential and apoptosis in ANKRD49 transfected group were significantly lower than those in other groups(P<0.05). Hoechst33258 staining results demonstrated that the percentage of apoptosis in ANKRD49 transfected group was significantly lower than that in other groups (P<0.05). Western blotting results showed that the expression of Cleaved-PARP and Cleaved-Caspase-3 protein in ANKRD49 over-expressed group was significantly lower than that in other tested groups (P<0.05), while the expression of Bcl-xL protein was significantly higher in ANKRD49 over-expressed group than that in control groups. Conclusion:Overexpression of ANKRD49 could inhibit the apoptosis of GC-1 cells via up-regulating Bcl-xL expression.
Key words: ANKRD49    Cell apoptosis    Bcl-xL    GC-1cells
收稿日期: 2016-10-25 出版日期: 2017-04-25
ZTFLH:  Q789  
通讯作者: 王海龙, 郭睿     E-mail: guoruipumc@aliyun.com;longwty@163.com
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引用本文:

白欣艳, 温丽敏, 王玉晶, 王海龙, 解军, 郭睿. ANKRD49通过上调Bcl-xL的表达抑制UV诱导GC-1细胞的凋亡[J]. 中国生物工程杂志, 2017, 37(4): 40-47.

BAI Xin-yan, WEN Li-min, WANG Yu-jing, WANG Hai-long, XIE Jun, GUO Rui. ANKRD49 Inhibits UV-induced Apoptosis of GC-1 Cells by Up-regulating Bcl-xL. China Biotechnology, 2017, 37(4): 40-47.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20170406        https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I4/40

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