Hmox1促进BMP9诱导C3H10T1/2细胞向成骨分化

doi:10.13523/j.cb.20170405

中国生物工程杂志

2017, Vol. 37

Issue (4): 33-39 DOI: 10.13523/j.cb.20170405

研究报告

Hmox1促进BMP9诱导C3H10T1/2细胞向成骨分化

刘晓骅, 吉彩霞, 徐丽, 董超群, 罗进勇

重庆医科大学检验医学院临床检验诊断学教育部重点实验室重庆 400016

Hmox1 Can Promote BMP9-induced Osteogenesis in C3H10 T1/2

LIU Xiao-hua, JI Cai-xia, XU Li, DONG Chao-qun, LUO Jin-yong

Key laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China

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摘要： 目的：研究血红素加氧酶1（hemeoxygenase 1，Hmox1）在骨形态发生蛋白9（bone morphogenetic proteins 9，BMP9）诱导下间充质干细胞C3H10T1/2向成骨分化过程中发挥的作用。方法：用Ad-BMP9感染C3H10T1/2，分别用Q-PCR和Western blot检测Hmox1mRNA和蛋白水平的变化；Hmox1激动剂COPP处理BMP9诱导的C3H10T1/2细胞，碱性磷酸酶（ALP）染色和活性测定检测早期成骨指标ALP的变化；过表达Hmox1的重组腺病毒（Ad-Hmox1）处理BMP9诱导的C3H10T1/2细胞，ALP染色和活性测定早期成骨指标ALP，茜素红染色检测晚期成骨指标钙盐沉积，Western blot检测成骨相关基因COL1A1。结果：Ad-BMP9感染C3H10T1/2后，Hmox1的mRNA及蛋白水平均升高；BMP9与Hmox1激动剂COPP联用与BMP9组相比ALP的活性增强；Ad-Hmox1可以增强BMP9诱导下C3H10T1/2细胞的ALP活性和钙盐沉积，以及成骨相关基因COL1A1表达。结论：Hmox1可以促进BMP9诱导下C3H10T1/2细胞的成骨分化。

关键词： T1/2; BMP9; 成骨分化; C3H10; 血红素加氧酶1

Abstract: Objective:To study the effect of Hmox1 on BMP9-induced osteogenesis in C3H10T1/2. Methods:First of all, C3H10T1/2 were treated with Ad-BMP9, then the transcription and translation level of Hmox1 in C3H10T1/2 were detected with Q-PCR and Western blot. Subsequently, C3H10T1/2 were treated with Hmox1 agonist COPP and BMP9 or treated with Ad-Hmox1 or/and BMP9, ALP activity was detected by quantitative and staining assay at 5 day, Calcium deposition was assayed by Alizarin Red Staining at 14 day. At last, expression of COL1A1 was measured by Western blot. Results:C3H10T1/2 infected by Ad-BMP9 results in elevated gene and protein level of Hmox1. Ad-Hmox1 and Hmox1 agonist could enhance BMP9-induced ALP activity in C3H10T1/2. The expression of COL1A1 and calcium deposition were increased by Hmox1. Conclusion:Hmox1 can promote BMP9-induced osteogenic differentiation in C3H10T1/2.

Key words: Morphogenetic protein 9 Osteogenic differentiation Bone C3H10T1/2 Hmox1

收稿日期: 2016-09-13 出版日期: 2017-04-25

ZTFLH:

Q819

基金资助: 国家自然科学基金资助项目（81272006）

通讯作者: 罗进勇 E-mail: luojinyong@sina.com

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引用本文:

刘晓骅, 吉彩霞, 徐丽, 董超群, 罗进勇. Hmox1促进BMP9诱导C3H10T1/2细胞向成骨分化[J]. 中国生物工程杂志, 2017, 37(4): 33-39.

LIU Xiao-hua, JI Cai-xia, XU Li, DONG Chao-qun, LUO Jin-yong. Hmox1 Can Promote BMP9-induced Osteogenesis in C3H10 T1/2. China Biotechnology, 2017, 37(4): 33-39.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20170405 或 https://manu60.magtech.com.cn/biotech/CN/Y2017/V37/I4/33

[1] Ferrari G, Cusella-De Angelis G, Coletta M, et al. Muscle regeneration by bone marrow-derived myogenic progenitors. Science (New York, N.Y.), 1998,279(5356):1528-1530.
[2] Mackay A M, Beck S C, Murphy J M, et al. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Engineering, 1998,4(4):415-428.
[3] Pittenger M F, Mackay A M, Beck S C, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999,284(5411):143-147.
[4] Dudley A T, Lyons K M, Robertson E J. A requirement for bone morphogenetic protein-7 during development of the mammalian kidney and eye. Genes Dev, 1995,9(22):2795-2807.
[5] Chen D, Zhao M, Mundy G R. Bone morphogenetic proteins. Growth Factors (Chur, Switzerland), 2004,22(4):233-241.
[6] Valentin-Opran A, Wozney J, Csimma C, et al. Clinical evaluation of recombinant human bone morphogenetic protein-2. Clin Orthop Relat Res, 2002,395:110-120.
[7] Ristiniemi J, Flinkkila T, Hyvonen P, et al. RhBMP-7 accelerates the healing in distal tibial fractures treated by external fixation. J Bone Joint Surg Br, 2007,89(2):265-272.
[8] Luu H H, Song W X, Luo X, et al. Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells. Journal of Orthopaedic Research,2007,25(5):665-677.
[9] Ryter S W, Alam J, Choi A M. Heme oxygenase-1/carbon monoxide:from basic science to therapeutic applications. Physiological Reviews, 2006,86(2):583-650.
[10] Komatsu D E, Hadjiargyrou M. Activation of the transcription factor HIF-1 and its target genes, VEGF, HO-1, iNOS, during fracture repair. Bone, 2004,34(4):680-688.
[11] 赵艳芳, 宋涛, 刘跃亮,等. RUNX2对BMP9诱导的间充质干细胞C3H10T1/2成骨分化的影响. 中国生物工程杂志, 2013, 33(2):21-26. Zhao Y F, Song T, Liu Y L, et al. Effects of RUNX2 on BMP9-induced osteogenic differentiation of C3H10T1/2 mesenchymal stem cells. China Biotechnology, 2013, 33(2):21-26.
[12] Kapturczak M H, Wasserfall C, Brusko T, et al. Heme oxygenase-1 modulates early inflammatory responses:evidence from the heme oxygenase-1-deficient mouse. Am J Pathol, 2004,165:1045-1053.
[13] Takahashi T, Morita K, Akagi R, et al. Heme oxygenase-1:a novel therapeutic target in oxidative tissue injuries. Curr Med Chem, 2004,11(12):1545-1561.
[14] Ke K, Safder M A, Sul O J, et al. Hemeoxygenase-1 maintains bone mass via attenuating a redox imbalance in osteoclast. Mol Cell Endocrinol, 2015,409:11-20.
[15] Sakamoto H, Sakai E, Fumimoto R, et al. Deltamethrin inhibits osteoclast differentiation via regulation of heme oxygenase-1 and NFATc1. Toxicol In Vitro, 2012,26(6):817-822.
[16] Barbagallo I, Vanella A, Peterson S J, et al. Overexpression of heme oxygenase-1 increases human osteoblast stem cell differentiation. J Bone Miner Metab, 2010,28(3):276-288.
[17] Zhang L F, Qi J, Zuo G, et al. Osteoblast-secreted factors promote proliferation and osteogenic differentiation of bone marrow stromal cells via VEGF/Heme-oxygenase-1 pathway. PLoS One, 2014,9(6):e99946.

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