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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2016, Vol. 36 Issue (4): 104-109    DOI: 10.13523/j.cb.20160415
技术与方法     
一种费氏丙酸杆菌细胞离位转化生产维生素B12的方法
杜崴1,2, 徐国霞1,2, 王自强2, 王云山2, 张利平1, 苏志国2
1. 河北大学生命科学学院 保定 071002;
2. 中国科学院过程工程研究所生化工程国家重点实验室 北京 100080
Production of Vitamin B12 by Propionibacterium freudenreichii Ex-situ Cell Transformation
DU Wei1,2, XU Guo-xia1,2, WANG Zi-qiang2, WANG Yun-shan2, ZHANG Li-ping1, SU Zhi-guo2
1. College of Life Science Hebei University, Baoding 071002, China;
2. The State Key Lab of Biochemical Engineering, The Institute of Process Engineering of the Chinese Academy of Sciences, Beijing 100080, China
 全文: PDF(581 KB)   HTML
摘要:

为建立费氏丙酸杆菌的半连续耦合发酵工艺,克服DMB对维生素B12连续发酵的不利影响,考察了费氏丙酸杆菌菌体细胞离位转化合成维生素B12的可行性,优化了其离位转化工艺,确定了最佳的转化时机、转化体系及DMB添加方式,具体如下:当发酵进行至84 h时,将发酵液离心,收集菌体,然后用离心上清液重悬菌体,配成5倍浓度的菌液,加入终浓度为4.5 mg/L的DMB,于30 ℃条件下转化48 h,维生素B12的产量达到108.06 mg/L,转化效率为2.26 mg/(Lh)。

关键词: 费氏丙酸杆菌维生素B12离位转化    
Abstract:

The semi-continuous coupling fermentation process can significantly improve the economical efficiency of Propionibacterium freudenreichii. In order to avoid the negative effect of DMB addition on Propionibacterium freudenreichii semi-continuous fermentation, the feasibility of synthesis of vitamin B12 by ex-situ cell transformation is investigated. Also, the ex-situ cell transformation process is optimized from the aspects of cell seperation time, transformation system and DMB addition mode. The details are as follows: when the fermentation process is carried out for 84 h, cells are removed from the fermentation broth by centrifugation. The supernatant is used to resuspended the cells and a 5 times concentration suspension is obtained. Then a final concentration of 4.5 mg/L DMB is added to synthesis vitamin B12 for 48 h at 30 ℃. The Vitamin B12 yield can reached 108.06 mg/L, with a conversion efficiency of 2.26 mg/(Lh).

Key words: Propionibacterium freudenreichii    Ex-situ cell transformation    Vitamin B12
收稿日期: 2015-12-23 出版日期: 2016-01-26
ZTFLH:  Q819  
基金资助:

国家重点基础研究发展计划(973)基金(2013CB733604)资助项目

通讯作者: 王云山     E-mail: yswang@ipe.ac.cn
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杜崴
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引用本文:

杜崴, 徐国霞, 王自强, 王云山, 张利平, 苏志国. 一种费氏丙酸杆菌细胞离位转化生产维生素B12的方法[J]. 中国生物工程杂志, 2016, 36(4): 104-109.

DU Wei, XU Guo-xia, WANG Zi-qiang, WANG Yun-shan, ZHANG Li-ping, SU Zhi-guo. Production of Vitamin B12 by Propionibacterium freudenreichii Ex-situ Cell Transformation. China Biotechnology, 2016, 36(4): 104-109.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160415        https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I4/104

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