赖氨酸酰化酶的重组表达及其催化合成ε-月桂酰-L-赖氨酸

doi:10.13523/j.cb.20160209

中国生物工程杂志

2016, Vol. 36

Issue (2): 62-67 DOI: 10.13523/j.cb.20160209

研究报告

赖氨酸酰化酶的重组表达及其催化合成ε-月桂酰-L-赖氨酸

成采虹, 杜婷, 陈可泉, 李艳

南京工业大学生物与制药工程学院南京 211816

Recombinant Expression of ε-Lysine Acylase from Streptomyces mobaraensis for Synthesis of Nε-lauroyl-L-lysine

CHENG Cai-hong, DU Ting, CHEN Ke-quan, LI Yan

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China

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摘要：

来源于链霉菌的赖氨酸酰化酶Sm-ELA能催化赖氨酸和月桂酸在水相中合成月桂酰赖氨酸,避免了采用化学合成法所必需的高温和有机溶剂条件,是一种节能、环境友好的替代方法。构建了过表达链霉菌赖氨酸酰化酶基因的重组质粒pET28a-SmELA和pTrcOmpXK₁₂₂SmELA,分别实现了该酶在大肠杆菌胞内和细胞表面的活性表达。比较两种不同表达方式的效果后,将重组酶应用于催化合成月桂酰赖氨酸的反应中,结果显示,在赖氨酸浓度为50 mmol/L,月桂酸浓度为10 mmol/L时,反应24 h,月桂酸转化率最高达到31.1%。

关键词： 酰基转移酶; 生物催化; 月桂酰赖氨酸

Abstract:

ε-Lysine acylase from Streptomyces mobaraensis(Sm-ELA) can catalyzes hydrolysis of Nε-lauroyl-L-lysine in water with ε-lysine and lauric acid as the substrates. It's an energy-saving and environmentally friendly way that avoids the high temperature and organic solvent by using the chemical method. Two recombinant plasmids which are pET28a-SmELA and pTrcOmpXK₁₂₂SmELA were constructed and a normal expression of intracellular and the cell surface were achieved respectively. The recombinant enzymes were used in catalytic synthesis of Nε-lauroyl-L-lysine and the catalytic efficiency was preliminarily compared. Nε-lauroyl-L-lysine was synthesized from 50 mmol/L ε-lysine and 10 mmol/L lauric acid in an aqueous buffer solution at 37℃. The maximum yield was 31. 1% after 24 h of reaction for 10 mmol/L lauric acid.

Key words: Nε-lauroyl-L-lysine Biocatalysis Acyl transferase

收稿日期: 2015-07-22 出版日期: 2015-12-22

ZTFLH:

Q789

基金资助:

国家"863"计划资助项目(2015AA021005)

通讯作者: 李艳 E-mail: liyan@njtech.edu.cn

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引用本文:

成采虹, 杜婷, 陈可泉, 李艳. 赖氨酸酰化酶的重组表达及其催化合成ε-月桂酰-L-赖氨酸[J]. 中国生物工程杂志, 2016, 36(2): 62-67.

CHENG Cai-hong, DU Ting, CHEN Ke-quan, LI Yan. Recombinant Expression of ε-Lysine Acylase from Streptomyces mobaraensis for Synthesis of Nε-lauroyl-L-lysine. China Biotechnology, 2016, 36(2): 62-67.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160209 或 https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I2/62

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