肾肿瘤相关基因的共表达网络构建与分析

doi:10.13523/j.cb.20160205

中国生物工程杂志

2016, Vol. 36

Issue (2): 30-37 DOI: 10.13523/j.cb.20160205

研究报告

肾肿瘤相关基因的共表达网络构建与分析

梁栋, 邢永强, 蔡禄

内蒙古科技大学数理与生物工程学院包头 014010

The Construction and Analysis of the Coexpression Network for the Gene Related to Renal Tumor

LIANG Dong, XING Yong-qiang, CAI Lu

School of Mathematics, Physics and Biological Engineering, Inner Mongolia University of Science and Technology, Baotou 014010, China

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摘要：

作为泌尿系统常见的肿瘤之一,肾肿瘤发病率在逐年上升。针对Affymetrix hgu133b的基因芯片数据进行差异表达基因筛选,应用加权基因共表达网络分析算法构建肾肿瘤差异表达基因的共表达网络。分析肾部正常组织和肿瘤组织差异表达基因之间的关联模式;选取与肿瘤发生关联程度最高的模块,筛选枢纽基因。最后,针对枢纽基因进行基因本体富集分析。细胞衰老是抑制肿瘤发生的主要机制之一,分析结果显示枢纽基因PLA2R1和TBX3与细胞衰老有关,可能对肾肿瘤的形成具有重要影响。该结果与基因PLA2R1通过促进细胞衰老抑制肾部肿瘤发生的研究结论一致。

关键词： 枢纽基因; WGCNA算法; 共表达网络; 肾肿瘤; GO分析

Abstract:

As one of common cancers in urinary system, the morbidity of renal tumor is increasing year by year. Firstly, the genes differentially expressed were screened based on Affymetrix hgu133b microarray data. The algorithm of Weighted Gene Co-expression Network Analysis was used to construct the co-expression network of the genes differentially expressed in renal tumor. Secondly, the module that is closely related with the tumor and hub genes were selected by analyzing the correlation patterns of the genes differentially expressed between normal and tumor tissues in kidney. Finally, the Gene Ontology enrichment of the hub genes was analyzed. Cell aging is one mechanism of suppressing tumorigenesis. The results indicated hub genes PLA2R1 and TBX3 are related to cell aging and could have an important effect on the tumor formation. The results corresponded with the investigation that gene PLA2R1 suppresses tumorigenesis in the kidney by promoting cellular senescence.

Key words: Renal tumor Co-expression network WGCNA Hub gene GO analysis

收稿日期: 2015-11-23 出版日期: 2015-12-14

ZTFLH:

Q819

基金资助:

国家自然科学基金面上项目(61271448; 11547150)、内蒙古自然科学基金面上项目(2014MS0311; 2014MS0312)资助项目

通讯作者: 蔡禄 E-mail: nmcailu@163.com

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引用本文:

梁栋, 邢永强, 蔡禄. 肾肿瘤相关基因的共表达网络构建与分析[J]. 中国生物工程杂志, 2016, 36(2): 30-37.

LIANG Dong, XING Yong-qiang, CAI Lu. The Construction and Analysis of the Coexpression Network for the Gene Related to Renal Tumor. China Biotechnology, 2016, 36(2): 30-37.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20160205 或 https://manu60.magtech.com.cn/biotech/CN/Y2016/V36/I2/30

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