技术与方法 |
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Primer Spanner:一个高效的定点突变PCR引物设计在线工具 |
裴智勇1,2, 侯仙慧3,4, 桂小柯1, 陈禹保1 |
1. 北京市科学技术研究院北京市计算中心 北京 100094;
2. 中国科学院北京基因组研究所 北京 100101;
3. 北京大学北京核磁共振中心 北京 100871;
4. 北京大学化学与分子工程学院 北京 100094 |
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Primer Spanner: A Web-based Platform to Design PCR Primers for High Efficient Site-directed Mutagenesis |
PEI Zhi-yong1,2, HOU Xian-hui3,4, GUI Xiao-ke1, CHEN Yu-bao1 |
1. Beijing Computing Center, Beijing Academy of Science and Technology, Beijing 100094, China;
2. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;
3. Beijing Nuclear Magnetic Resonance Center, Peking University, Beijing 100871, China;
4. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
引用本文:
裴智勇, 侯仙慧, 桂小柯, 陈禹保. Primer Spanner:一个高效的定点突变PCR引物设计在线工具[J]. 中国生物工程杂志, 2015, 35(10): 53-58.
PEI Zhi-yong, HOU Xian-hui, GUI Xiao-ke, CHEN Yu-bao. Primer Spanner: A Web-based Platform to Design PCR Primers for High Efficient Site-directed Mutagenesis. China Biotechnology, 2015, 35(10): 53-58.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20151008
或
https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I10/53
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