PolyI:C协同IL-15作为小鼠卵透明带3基因疫苗佐剂黏膜免疫效果的观察

doi:10.13523/j.cb.20150902

中国生物工程杂志

2015, Vol. 35

Issue (9): 7-13 DOI: 10.13523/j.cb.20150902

研究报告

PolyI:C协同IL-15作为小鼠卵透明带3基因疫苗佐剂黏膜免疫效果的观察

王丹阳¹, 杨雨¹, 杨秀梅¹, 张富春¹, 吴道澄², 张爱莲¹

1 新疆大学生命科学与技术学院乌鲁木齐 830046;
2 西安交通大学生命科学与技术学院西安 710049

The Efficacy of PolyI:C and IL-15 as Adjuvant the Murine Zona Pellucid 3 DNA Vaccine Intranasally Immunized

WANG Dan-yang¹, YANG Yu¹, YANG Xiu-mei¹, ZHANG Fu-chun¹, WU Dao-cheng², ZHANG Ai-lian¹

1 College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;
2 School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China

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摘要：

目的:以Toll样受体拮抗物PolyI:C协同细胞因子IL-15作为免疫佐剂,通过滴鼻方式免疫ICR小鼠,对卵透明带3(murine zona pellucida,mZP3)基因疫苗的免疫增强效果进行观察.方法:将PolyI:C和IL-15单独或协同与用壳聚糖(chitosan,Chi)包裹的 mZP3 基因疫苗滴鼻免疫ICR小鼠,ELISA法检测小鼠血清中特异性抗mZP3抗体和生殖道黏液、肺洗液中sIgA的水平,MTT法检测淋巴细胞增殖,小鼠的平均窝仔数和生育率检测抗生育情况,组织学观察小鼠的卵巢病理切片.结果:PolyI:C协同IL-15共同免疫组能够显著提高免疫后小鼠抗mZP3抗体的水平,促进淋巴细胞的增殖,平均窝仔数和生育率显著降低,小鼠的卵巢发育异常.结论:PolyI:C协同IL-15能够增强 mZP3 基因疫苗的免疫效果,并在一定程度上降低小鼠生育率.

关键词： PolyI:C; IL-15; 基因疫苗; 佐剂

Abstract:

Aim:To observe the adjuvant efficacy of murine zona pellucid 3(mZP3) DNA vaccine through intranasal vaccination with PolyI:C and IL-15, and chitosan as delivery vector. Methods: Mice were delivered with chitosan-loaded mZP3 DNA vaccine or added PolyI:C and IL-15 by intranasal route, and to detect specific IgG antibody level in serum, sIgA in vaginal fluid and lung fluid by indirect ELISA, to detect lymphocyte proliferation by MTT, to do histology analysis of mice ovary for morphology of ovary. Results: PolyI:C and IL-15 can promote IgG antibody level of immunized mZP3 mice, promote lymphocyte proliferation and ovarian abnormalities in mice. Conclusion: The results suggest that antagonist of TLR PolyI:C and IL-15 have a synergistic effect and may be enhance the immunity effect of mZP3 DNA vaccine and reduce murine fertility rate to some extent.

Key words: PolyI:C IL-15 DNA vaccine Adjuvant

收稿日期: 2015-04-10 出版日期: 2015-09-25

ZTFLH:

R392.12

基金资助:

新疆生物资源基因工程重点实验室开放课题资助项目(XJDX0201-2013-07)

通讯作者: 张爱莲 E-mail: xjzal@163.com

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引用本文:

王丹阳, 杨雨, 杨秀梅, 张富春, 吴道澄, 张爱莲. PolyI:C协同IL-15作为小鼠卵透明带3基因疫苗佐剂黏膜免疫效果的观察[J]. 中国生物工程杂志, 2015, 35(9): 7-13.

WANG Dan-yang, YANG Yu, YANG Xiu-mei, ZHANG Fu-chun, WU Dao-cheng, ZHANG Ai-lian. The Efficacy of PolyI:C and IL-15 as Adjuvant the Murine Zona Pellucid 3 DNA Vaccine Intranasally Immunized. China Biotechnology, 2015, 35(9): 7-13.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150902 或 https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I9/7

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