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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2015, Vol. 35 Issue (7): 83-93    DOI: 10.13523/j.cb.20150712
综述     
小鼠和大鼠的胚胎培养基及若干相关问题
高珊1, 陈炜1, 于磊1, 李静1, 孙彩显1, 高杰1, 刘牧1,2
1. 中国医学科学院中国医学实验动物研究所 北京 100021;
2. 中国疾病预防控制中心 北京 100050
Culturing of Mouse and Rat Preimplantation Embryos in Various Chemically Defined Media
GAO Shan1, CHEN Wei1, YU Lei1, LI Jing1, SUN Cai-xian1, GAO Jie1, LIU Mu1,2
1. Institute of Laboratory Animal Sciences, CAMS&PUMC, Beijing 100021, China;
2. Chinese Center for Disease Control and Prevention, Beijing 100050, China
 全文: PDF(545 KB)   HTML
摘要:

哺乳动物早期胚胎体外培养所用各种化学成份明确的培养基是研究生命科学中一项重要技术。它已被常规用于研究胚胎早期发育,多种动物及人类辅助生殖技术,转基因动物,动物克隆等领域。介绍了用于移植前胚胎培养基的研究和发展历史,当前所用化学成份明确胚胎培养基的主要组成,特别是针对小鼠和大鼠移植前胚胎所用各种培养基及其成份,讨论了这类培养基发展前景和研究方向。

关键词: 移植前胚胎体外成熟体外受精体外培养培养基化学成份明确的培养基    
Abstract:

The mammalian oocytes were in vitro fertilization (IVF), the preimplantation embryos (PE) were in vitro cultured (IVC) that was one of important technical in the modern bio-science. The normally to be used medium, one had been called chemically defined medium (CDM). It had been used in a wildly field, frequently. It included in the animal and human's artificial assisted reproductive technical (ART), to research the embryo of earlier developing stage, the transgenic animal, and the animals clone, etc. The review was included in the development history and its constituents of the CDM. There was also included some modified embryo media, and focused on the especial media, which was to be used for culturing the mouse or rat embryo. Meanwhile, the article had introduced the prospects and research direction of CDM, briefly.

Key words: Preimplantation embryo    In vitro maturation    In vitro fertilization    In vitro culture    Medium    Chemically defined medium
收稿日期: 2015-03-23 出版日期: 2015-07-25
ZTFLH:  Q78  
基金资助:

国家科技支撑计划资助项目(2014BA202B01)

通讯作者: 刘牧     E-mail: liumu_cn@163.com
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引用本文:

高珊, 陈炜, 于磊, 李静, 孙彩显, 高杰, 刘牧. 小鼠和大鼠的胚胎培养基及若干相关问题[J]. 中国生物工程杂志, 2015, 35(7): 83-93.

GAO Shan, CHEN Wei, YU Lei, LI Jing, SUN Cai-xian, GAO Jie, LIU Mu. Culturing of Mouse and Rat Preimplantation Embryos in Various Chemically Defined Media. China Biotechnology, 2015, 35(7): 83-93.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150712        https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I7/83


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