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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2015, Vol. 35 Issue (2): 92-98    DOI: 10.13523/j.cb.20150214
综述     
转基因动物乳腺生物反应器相关技术及研究进展
梁振鑫1, 尹富强1, 刘庆友2, 李力1
1. 广西医科大学 南宁 530021;
2. 广西大学 南宁 530004
Progress and Technology in Mammary Gland Bioreactor of Transgenic Animals
LIANG Zhen-xin1, YIN Fu-qiang1, LIU Qing-you2, LI Li1
1. Guangxi Medical University, Nanning 530021, China;
2. Guangxi University, Nanning 530004, China
 全文: PDF(442 KB)   HTML
摘要:

转基因动物乳腺生产人类重组蛋白人血清白蛋白(hSA)、人纤维蛋白原(hFIB)、人蛋白C (hPC)、人凝血因子(hF-Ⅷ) 和(hF-Ⅸ)等具有较高市场潜力,使其在医学领域获得广泛应用。对转基因动物乳腺生物反应器的优势、目的基因选择、载体构建、基因工程技术、重组蛋白在肿瘤治疗上的应用、当前困境和未来前景等方面进行较为全面的综述,以期能有助于转基因动物生物反应器的研究。此外较详细地探讨了利用 CRISPR-Cas基因打靶技术生产高表达量的人类重组蛋白的可能性。

关键词: 动物乳腺生物反应器载体构建转基因技术生物制药    
Abstract:

Production of human serum albumin (hSA), fibrinogen (hFIB), human protein c (hPC), human blood clotting factor VIII (HF-VIII)and human blood clotting factor (hF-IX) by mammary gland bioreactor of transgenic has great market potential and is widely used in medical areas. A comprehensive review was made on the advantages, selection of target genes, construction of vector, genetic engineering technology, the application of recombinant proteins in cancer treatment, problems and prospects of mammary gland bioreactor of transgenic animals, in order to contribute to the study on transgenic animal bioreactors. Also the possibility of high level expression of human recombinant proteins by the method of CRISPR-Cas gene targeting was discussed.

Key words: Mammary gland bioreactor    Vector construction    Transgenic technology    Bio-pharmacy
收稿日期: 2014-12-03 出版日期: 2015-02-25
ZTFLH:  Q819  
通讯作者: 李力     E-mail: lili@gxmu.edu.cn
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引用本文:

梁振鑫, 尹富强, 刘庆友, 李力. 转基因动物乳腺生物反应器相关技术及研究进展[J]. 中国生物工程杂志, 2015, 35(2): 92-98.

LIANG Zhen-xin, YIN Fu-qiang, LIU Qing-you, LI Li. Progress and Technology in Mammary Gland Bioreactor of Transgenic Animals. China Biotechnology, 2015, 35(2): 92-98.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20150214        https://manu60.magtech.com.cn/biotech/CN/Y2015/V35/I2/92


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