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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2014, Vol. 34 Issue (9): 108-112    DOI: 10.13523/j.cb.20140916
综述     
细菌菌影作为新型疫苗和递送载体的研究进展
杨慧, 焦红梅
扬州大学医学院 病原生物学与免疫学教研室 扬州 225001
Advances on Bacterial Ghost as Novel Vaccine and Delivery Vector
YANG Hui, JIAO Hong-mei
Medical College of Yangzhou University, Yangzhou 225001, China
 全文: PDF(383 KB)   HTML
摘要:

细菌菌影(bacterial ghost,BG)是革兰阴性菌在噬菌体PhiX174的裂解基因E的作用下形成不含核酸、核糖体等胞质内容物的细菌空壳。这种细菌空壳保留了与天然细菌一样的完整外膜结构,且不具有活菌样的致病作用,可作为疫苗无需佐剂就能诱导机体产生体液免疫应答和细胞免疫应答。菌影内部及外膜上可装载DNA、抗原和药物等异源物质,易被机体免疫细胞识别捕获,使其成为一种新型的生物递送载体。另外,菌影具有制备简单,易于保存等优点。细菌菌影在疾病预防和治疗方面具有广阔的应用前景。

关键词: 细菌菌影疫苗递送载体    
Abstract:

Bacterial ghost (BG) is an empty bacterial envelope of gram-negative bacteria devoid of cytoplasmic content,and produced by controlled expression of bacteriophage PhiX174 lysis gene E. BG keeps intact bacterial outer membrane structure, such as flagella, LPS and outer membrane proteins, making bacterial easy to adherence to the human or animal cell receptors, so it can be used as vaccines without adjuvant to induce strong humoral immune response and cellular immune response. BG has many spaces, such as the outer membrane, the periplasmic space and the inner membrane to load other substance. BG also can be used as DNA, antigen and drugs delivery vector as it exhibits potency with regard to target antigens and large capacity compared to conventional approaches. In addition, BG can be provided with a foreign antigen, nucleic acid or other ingredients to construct combined vaccine for human or animal health services. BG has wide application prospect in the prevention and treatment of disease.

Key words: Bacterial ghost    Vaccine    Delivery vector
收稿日期: 2014-07-08 出版日期: 2014-09-25
ZTFLH:  Q78  
基金资助:

国家自然科学基金资助项目(31100652)

通讯作者: 焦红梅     E-mail: hmjiao@yzu.edu.cn
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引用本文:

杨慧, 焦红梅. 细菌菌影作为新型疫苗和递送载体的研究进展[J]. 中国生物工程杂志, 2014, 34(9): 108-112.

YANG Hui, JIAO Hong-mei. Advances on Bacterial Ghost as Novel Vaccine and Delivery Vector. China Biotechnology, 2014, 34(9): 108-112.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140916        https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I9/108


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