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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2014, Vol. 34 Issue (8): 105-111    DOI: 10.13523/j.cb.20140816
综述     
枯草芽胞杆菌芽胞表面展示研究进展
谢志丹1, 范稳1, 贾东晨1, 杨娜1, 夏振远2, 乔敏1
1. 云南大学微生物保护与利用重点实验室 昆明 650091;
2. 云南省烟草农业科学研究院 昆明 650091
Recent Developments in Spore Surface Display of Bacillus subtilis
XIE Zhi-dan1, FAN Wen1, JIA Dong-cheng1, YANG Na1, XIA Zheng-yuan2, QIAO Min1
1. Key Laboratory for Conservation and Utilization of Bio-Resource, Yunnan University, Kunming 650091, China;
2. Yunnan Tobacco Research Institute of Agricultural Science, Kunming 650091, China
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摘要：

枯草芽胞杆菌芽胞表面展示技术是把枯草芽胞杆菌作为芽胞表面展示的宿主来展示目的蛋白的一种技术。该技术不仅具备芽胞表面展示技术可展示分子量较大的目的蛋白、目的蛋白无需跨膜及芽胞的极强抗逆性等特点外，同时由于该技术的宿主菌——枯草芽胞杆菌的分子生物学信息研究得比较清楚、安全性高而被广泛应用。介绍了枯草芽胞杆菌表面展示近10年在生产疫苗和固定化酶方面的进展，并对如何提高表面展示目的蛋白的产量做了简要概述。
关键词： 表面展示;  枯草芽胞杆菌;  抗原;  锚定蛋白    
Abstract:

Bacillus subtilis spore display, a technique that presents proteins or peptides on the surface of the spore of Bacillus subtilis, enables high-throughput screening and has become an essential tool in bimolecular engineering. The major advantage of spores over the other published systems is their synthesis within the cytoplasm of the bacterial cell. Therefore, any heterologous protein to be anchored on the outside does not have to cross any membrane. Furthermore,spores are extremely resistant against high temperature, irradiation and many chemicals, and can be stored for many years at room temperature. With the advantage of complete genome information and security, Bacillus subtilis spore display now is attracting more and more attention. The following describes the recent progress of surface of Bacillus subtilis in the produce of vaccines and the immobillzed enzyme, and present a brief overview about how to improve the yield of the target protein surface display.
Key words: Surface display    Bacillus subtilis    Antigen    Anchoring protein
收稿日期: 2014-05-23 出版日期: 2014-08-25
ZTFLH:  Q819  
基金资助:

国家自然科学基金（31360130）、云南省烟草公司科技计划项目（2012YN06）资助项目
通讯作者: 乔敏，E-mail：mingqiao@ynu.edu.cn     E-mail: mingqiao@ynu.edu.cn
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引用本文:

谢志丹, 范稳, 贾东晨, 杨娜, 夏振远, 乔敏. 枯草芽胞杆菌芽胞表面展示研究进展[J]. 中国生物工程杂志, 2014, 34(8): 105-111.

XIE Zhi-dan, FAN Wen, JIA Dong-cheng, YANG Na, XIA Zheng-yuan, QIAO Min. Recent Developments in Spore Surface Display of Bacillus subtilis. China Biotechnology, 2014, 34(8): 105-111.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140816    或    https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I8/105


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