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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2014, Vol. 34 Issue (4): 46-52    DOI: 10.13523/j.cb.20140408
研究报告     
乙、丁酸添加条件下丁醇发酵图论模型的构建
李志刚, 李鑫, 史仲平
江南大学生物工程学院 工业生物技术教育部重点实验室 无锡 214122
Model Construction for Butanol Fermentations with Acetate/Butyrate Added by Graph Theory
LI Zhi-gang, LI Xin, SHI Zhong-ping
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
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摘要：

有机酸代谢途径在丁醇发酵过程中具有重要的作用，对细胞内碳流的分配和产物的合成影响显著。在7 L厌氧发酵罐中，进行了间歇添加乙酸或丁酸的发酵实验。结果表明，乙、丁酸的添加显著提高了总溶剂的生产效率，分别提高了47.1%和39.2%；此外，丁醇/丙酮比在添加丁酸的批次中提高了21.7%，在添加乙酸的批次中降低了16.2%；厌氧瓶中的发酵实验也证实了以上结果。有机酸代谢计算的结果表明，乙、丁酸的添加基本上阻断了相应有机酸闭环的吸收途径。基于相关报道和代谢计算结果，构建了针对乙、丁酸添加批次的图论模型，并利用该模型对不同发酵条件下的溶剂浓度和丁醇/丙酮比进行了计算。结果表明，该模型很好地预测了实验结果，合理地构建了乙、丁酸添加批次的信号传递线图。
关键词： 丁醇发酵;  模型构建;  图论;  生产效率;  丁醇/丙酮比    
Abstract:

Acetate/butyrate metabolic pathways play an important role in ABE fermentation and their changes will influence entire carbon fluxes distribution. Several fermentations with intermittent feeding of acetate/butyrate were conducted in a 7 L fermentor and the results indicated that exogenous acetate/butyrate enhanced solvents productivities by 47.1% and 39.2% respectively, and butanol/acetone ratios were improved by 21.7% for butyrate added batch and decreased by 16.2% for acetate added batch. A nonlinear constraint was utilized for acids rates calculation and the results revealed that acetate/butyrate formation pathways were almost blocked by corresponding acids feeding. Then a metabolic model constructed by graph theory was utilized to dispose those cases with acetate/butyrate added and to predict solvents production, in which some improvements were adopted based on the calculation results and related references. The model predicted solvents production, butanol/acetone ratios accurately and constructed the directed signal flow diagrams of ABE network under different conditions correctly.
Key words: Butanol fermentation    Model construction    Graph theory    Solvents productivities    Butanol/acetone ratio
收稿日期: 2014-01-03 出版日期: 2014-04-25
ZTFLH:  Q935  
基金资助:

国家自然科学基金资助项目（20976072）
通讯作者: 史仲平     E-mail: lp19890604@163.com
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引用本文:

李志刚, 李鑫, 史仲平. 乙、丁酸添加条件下丁醇发酵图论模型的构建[J]. 中国生物工程杂志, 2014, 34(4): 46-52.

LI Zhi-gang, LI Xin, SHI Zhong-ping. Model Construction for Butanol Fermentations with Acetate/Butyrate Added by Graph Theory. China Biotechnology, 2014, 34(4): 46-52.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140408    或    https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I4/46


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[1] . 改善抗体生产效率[J]. 中国生物工程杂志, 1985, 5(3): 77-77.
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