多孔陶瓷吸附固定纤维堆囊菌发酵制备埃博霉素

doi:10.13523/j.cb.20140316

中国生物工程杂志

2014, Vol. 34

Issue (3): 109-113 DOI: 10.13523/j.cb.20140316

技术与方法

多孔陶瓷吸附固定纤维堆囊菌发酵制备埃博霉素

龚国利, 刘丽丽, 王娜

陕西科技大学生命科学与工程学院西安 710021

The Sorangium Cellulosum Immobilized by Porous Ceramic Fermentation for Epothilone Preparation

GONG Guo-li, LIU Li-li, WANG Na

College of life science and engineering, Shaanxi university of science&technology, Xi'an 710021, China

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摘要： 由纤维堆囊菌产生的埃博霉素具有极大药用价值，但因纤维堆囊菌液体环境中聚团非均匀生长限制了埃博霉素的大规模生产。借助多孔陶瓷的多孔结构，可为粘细菌提供固体附着生长面，提高埃博霉素产量。利用造孔剂法制备硅藻土基多孔陶瓷，在优化制备及改性条件后，当30目木屑造孔剂用量为2.5%（质量分数），7 MPa下制得的硅藻土基多孔陶瓷性能良好，孔径集中在5 μm，比表面积为23.55 m2/g，孔隙率为32%，机械强度为10.2 MPa；经1.5 mol/L FeCl3改性的多孔陶瓷对纤维堆囊菌的吸附量达36.8 mg/g。在优化固定化发酵条件后，当在300 ml三角瓶中装液量为45 ml，固液比3：5，接种量10%，温度30 ℃，转速220 r/min，最初pH 7.5，发酵时间为8 d时，埃博霉素的产量达90.2 mg/L，与游离发酵相比提高了近4倍。

关键词： 纤维堆囊菌; 埃博霉素; 多孔陶瓷

Abstract: The Epothilone,produced by Sorangium cellulosum,has great medicinal value,however,the change of growth form on Sorangium cellulosum has limited the mass produce of Epothilone in the liquid circumstance.the porous structure of porous ceramic can provide a solid attachment and growth surface,then improve the production of Epothilone. The diatomite-based porous ceramics was made from diatomite by adding pore-making agent method.After optimized the preparation and modified conditions,it is found that best prescription contains 2.5% of 30 mesh pore-forming agent of wood powder(mass fraction),the forming pressure at 7MPa,the performance of porous ceramics are pore diameter 5 μm,specific surface 23.55 m²/g,porosity 32% and mechanical strength 10.2MPa.The adsoption capacity of porous ceramics,which is modified by 1.5mol/L FeCl₃,is 36.8mg/g.The optimal immobilized fermentation condition is:the liquid volum 45mL in the 300mL flask,the porous ceramics 2.7g modified by 1.5mol/L FeCl₃, inoculum 10%, temperature 30℃,shaking speed 220r/min,initial pH 7.5 and fermentation time 8d. the Epothilone production reached 90.2mg/L,which is increased 4 times.

Key words: Sorangium cellulosum Epothilone Porous ceramic Modified

收稿日期: 2013-12-16 出版日期: 2014-03-25

ZTFLH:

Q93-335

基金资助: 国家自然科学基金资助项目（20906058）

通讯作者: 龚国利 E-mail: gonguoli@sust.edu.cn

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引用本文:

龚国利, 刘丽丽, 王娜. 多孔陶瓷吸附固定纤维堆囊菌发酵制备埃博霉素[J]. 中国生物工程杂志, 2014, 34(3): 109-113.

GONG Guo-li, LIU Li-li, WANG Na. The Sorangium Cellulosum Immobilized by Porous Ceramic Fermentation for Epothilone Preparation. China Biotechnology, 2014, 34(3): 109-113.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140316 或 https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I3/109

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