电容法在线测定发酵过程中毕赤酵母浓度的研究

doi:10.13523/j.cb.20140313

中国生物工程杂志

2014, Vol. 34

Issue (3): 91-95 DOI: 10.13523/j.cb.20140313

技术与方法

电容法在线测定发酵过程中毕赤酵母浓度的研究

李兰¹, 王泽建¹, 金勇², 孙文华², 庄英萍¹, 张嗣良¹

1. 华东理工大学上海 201424;
2. 浙江谱创仪器有限公司

Study on On-line Capacitance Measurement to Evaluate the Viable Biomass During the Fermentation of Pichia

LI Lan¹, WANG Ze-Jian¹, JIN Yong², SUN Wen-hua², ZHUANG Ying-ping¹, ZHANG Si-liang¹

1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai China;
2. Zhejiang Puchuang Instrument Co., LTD, Shanghai, China

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摘要： 活细胞量是与细胞生长、代谢、生产力相关的重要生理参数。在线活细胞测定仪能够利用电容的原理检测发酵液中的活细胞量。本文详细介绍了国产在线活细胞测定仪的检测原理，并说明了其在毕赤酵母发酵过程中的应用情况。结果表明，我们设计的在线活细胞测定仪能够直接、实时、在线获得发酵过程中可靠的活细胞量，且有效避免采样操作的干扰。这一技术符合过程分析技术（PAT）的要求，并可以与其他参数相结合进行分析，作为发酵过程开发、控制和优化的有利工具。

关键词： 在线活细胞测定仪; 活细胞量; 在线电容测量; 毕赤酵母; PAT

Abstract: The amount of viable biomass is an important physiological parameter, which is correlated with the cell growth, metabolism and productivity in fermentations. Biomass Monitor is becoming an established tool for monitoring the capacitance to evaluate the concentration of viable biomass in the cell suspension. In this paper, the principle of the Biomass Monitor and the application in the Pichi fermentation is described. The results show that Biomass Monitor can directly obtain reliable amount of viable biomass in real-time during the fermentation process, and effectively avoid the interference of taking samples. This technology meets Process Analytical Technology (PAT) requirements, and it can be a useful tool combined with other data for the development, optimization, monitoring and control in fermentation processes.

Key words: Biomass monitor Viable biomass On-line capacitance measurement Pichia PAT

收稿日期: 2013-10-21 出版日期: 2014-03-25

ZTFLH:

Q815

基金资助: 国家科技支撑计划资助项目（2011BAF02B03）

通讯作者: 庄英萍 E-mail: ypzhuang@ecust.edu.cn

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引用本文:

李兰, 王泽建, 金勇, 孙文华, 庄英萍, 张嗣良. 电容法在线测定发酵过程中毕赤酵母浓度的研究[J]. 中国生物工程杂志, 2014, 34(3): 91-95.

LI Lan, WANG Ze-Jian, JIN Yong, SUN Wen-hua, ZHUANG Ying-ping, ZHANG Si-liang. Study on On-line Capacitance Measurement to Evaluate the Viable Biomass During the Fermentation of Pichia. China Biotechnology, 2014, 34(3): 91-95.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140313 或 https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I3/91

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