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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2014, Vol. 34 Issue (3): 56-60    DOI: 10.13523/j.cb.20140308
研究报告     
bmy基因敲除对解淀粉芽孢杆菌Q-426溶血性及抗菌活性的影响
陶思美1,2, 郑维1,2, 赵朋超3, 周伟1,2, 权春善1,2, 范圣第1,2
1. 大连民族学院生命科学学院 大连 116600;
2. 国家民委教育部重点实验室 大连 116600;
3. 河南科技大学医学技术与工程学院 洛阳 471023
Effects of bmy Gene knockout on Hemolysis and Antifungal activity of Bacillus amyloliquefaciens Q-426
TAO Si-mei1,2, ZHENG Wei1,2, ZHAO Peng-chao3, ZHOU Wei1,2, QUAN Chun-shan1,2, FAN Sheng-di1,2
1. Department of Life Science, Dalian Nationalities University, Dalian 116600, China;
2. SEACME Key laboratory, Dalian 116600, China;
3. Department of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471003, China
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摘要： Iturin家族环脂肽对于红色毛癣菌等病原真菌具有较强的抑菌活性，有潜力成为治疗皮肤疾病的新型抗真菌药物。解淀粉芽孢杆菌Q-426菌株能够产生环脂肽fengycins和iturin家族的bacillomycin D，但该菌株发酵液具有溶血活性。为确定bacillomycin D是否为引发溶血作用的主要物质，本文利用同源重组基因敲除技术，构建解淀粉芽孢杆菌bmy基因缺失菌株，抑制bacillomycin D的合成，研究对其溶血性及抗菌活性的影响。突变菌株发酵液中未检测到bacillomycin D产生，且发酵液的溶血性及抗菌活性明显减小，表明bacillomycin D与该菌的溶血活性及抑菌活性密切相关。
关键词： 环脂肽;  基因敲除;  bmy基因;  溶血性;  抗菌活性    
Abstract: Iturin family of lipopeptides has strong antifungal activity against pathogenic fungi such as Trichophyton rubrum and has potential to become a new antifungal drug currently used against dermatophytes. Bacillus amyloliquefaciens Q-426 could produce fengycins and bacillomycin D (a member of iturin family), and its fermentation broth shows a hemolytic activity. In order to determine whether bacillomycin D is the main hemolytic substances, a homologous recombinantion gene knockout technology was used to construct strain Q-426-Δ-bmy. Bacillomycin D was not detected in the Q-426-Δ-bmy strain fermentation broth, and there was a significant reduction in the hemolysis and antifungal activity in contrast with wild strain Q-426, indicating that bacillomycin D is closely related to hemolytic activity and antifungal activity of Bacillus amyloliquefaciens Q-426.
Key words: Cyclic lipopeptides    Gene knockout    bmy gene    Hemolysis    Antifungal activity
收稿日期: 2013-12-17 出版日期: 2014-03-25
ZTFLH:  Q78  
基金资助: 辽宁省教育厅重点实验室资助项目（LS2010049）
通讯作者: 权春善     E-mail: mikyeken@dlnu.edu.cn
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引用本文:

陶思美, 郑维, 赵朋超, 周伟, 权春善, 范圣第. bmy基因敲除对解淀粉芽孢杆菌Q-426溶血性及抗菌活性的影响[J]. 中国生物工程杂志, 2014, 34(3): 56-60.

TAO Si-mei, ZHENG Wei, ZHAO Peng-chao, ZHOU Wei, QUAN Chun-shan, FAN Sheng-di. Effects of bmy Gene knockout on Hemolysis and Antifungal activity of Bacillus amyloliquefaciens Q-426. China Biotechnology, 2014, 34(3): 56-60.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140308    或    https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I3/56

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