技术与方法适体核酶型人工核糖开关的设计

doi:10.13523/j.cb.20140210

中国生物工程杂志

2014, Vol. 34

Issue (2): 59-64 DOI: 10.13523/j.cb.20140210

技术与方法

技术与方法适体核酶型人工核糖开关的设计

王佳稳^1,2, 冯婧娴^1,2, 林俊生^1,2, 刁勇^1,2

1. 华侨大学分子药物研究院泉州 362021;
2. 华侨大学生物医学学院泉州 362021

The Artificial Aptazyme Based Riboswitch

WANG Jia-wen^1,2, FENG Jing-xian^1,2, LIN Jun-sheng^1,2, DIAO Yong^1,2

1. Institute of Molecular Medicine, Quanzhou 362021 China;
2. School of Biomedical Sciences Huaqiao University, Quanzhou 362021, China

全文: PDF(750 KB) HTML

摘要： 适体核酶型核糖开关是近年出现的一种人工基因调控开关。最常见的适体核酶由锤头状核酶和适体组成，结构清晰，易于设计。作为一种顺式作用元件，适体核酶型核糖开关在特异性配体的作用下，无需蛋白质辅助，即可通过调节自身裂解反应，调控mRNA的翻译，可应用于多种细胞的基因调控。目前适体核酶型核糖开关的设计，主要是通过合理组装核酶与适体元件，整合到mRNA后再进行功能筛选。该基因调控开关调节幅度大、响应迅速、调控方式简洁，有可能应用于体内传感器、基因治疗、生物处理器等多个领域。

关键词： 适体; 模块组装; 基因调控; 锤头状核酶

Abstract: The artificial aptazyme based riboswitch has emerged as a powerful tool for regulating gene expression in recent years. The most common aptazyme consists of a hammerhead ribozyme and an aptamer, it is simply a piece of structured RNA and easy to design. As a cis-acting element, aptazyme riboswitch can recognize specific targets and exert effective influence on translation without the help of protein co-factor through irreversible self-cleavage in vivo, which makes it a versatile platform functions in various cells. The most popular approach to generate an aptazme based riboswitch is to rationally cojoin a ribozyme to an aptamer and screen with a high throughout method after integrated into mRNA. With considerable magnitude of on-off control and rapid induction as well as concise regulation, this riboswitch will have important application in in vivo sensing, gene therapy and biological processor.

Key words: Aptamer Modular assembly Gene regulation Hammerhead ribozyme

收稿日期: 2013-12-03 出版日期: 2014-02-25

ZTFLH:

Q789

基金资助: 国家自然科学基金（81271691，81371669）、国际科技合作项目（2011DFG33320）、福建省科技重大项目（2013N5007）资助项目

通讯作者: 刁勇 E-mail: diaoyong@hqu.edu.cn

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引用本文:

王佳稳, 冯婧娴, 林俊生, 刁勇. 技术与方法适体核酶型人工核糖开关的设计[J]. 中国生物工程杂志, 2014, 34(2): 59-64.

WANG Jia-wen, FENG Jing-xian, LIN Jun-sheng, DIAO Yong. The Artificial Aptazyme Based Riboswitch. China Biotechnology, 2014, 34(2): 59-64.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140210 或 https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I2/59

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