中国生物工程杂志

To explore the expression of miR-5047 in breast cancer cells and its role in breast cancer cell proliferation and migration, and to clarify the role of decitabine (DAC) in the regulation of miR-5047 expression. The expression level of miR-5047 in human breast cancer cell lines and normal breast epithelial cells MCF10A was detected by real-time quantitative PCR (qRT-PCR). Transfect miR-5047 mimic and negative control mimic NC into MDA-MB-231 and MCF7 cells, respectively, and verify the transfection efficiency by qRT-PCR. Plate cloning experiments, MTT experiments, and scratch healing experiments were used to detect the proliferation and migration ability of breast cancer cells, and qRT-PCR and Western blot methods were used to detect the expression of related genes and proteins after over-expression of miR-5047. MDA-MB-231 and MCF-7 cells were treated with DAC at final concentrations of 5 μmol/L and 10 μmol/L, and qRT-PCR was used to detect the effect of DAC on miR-5047 expression under different concentrations and treatment time. At the same time, the effect of DAC on the epithelial mesenchymal transition (EMT) of breast cancer cells was detected by morphological observation and Western blot. Compared with normal breast epithelial cells MCF-10A, the expression of miR-5047 in breast cancer cells was significantly down-regulated. Overexpression of miR-5047 can significantly inhibit the proliferation and migration of breast cancer cells, promote the expression of epithelial cell marker E-cadherin, and inhibit the expression of mesenchymal cell marker Vimentin. The expression of miR-5047 can promote the expression of epithelial cell marker E-cadherin and inhibit the expression of mesenchymal cell marker Vimentin. After treating MDA-MB-231 and MCF7 cells with different concentrations of DAC, the expression of miR-5047 was enhanced, and the effect was most significant when 10 μmol/L DAC was used for 48 h. DAC can induce epithelial transformation of MDA-MB-231 cells. The expression of miR-5047 is significantly down-regulated in breast cancer cell lines. Overexpression of miR-5047 can inhibit the proliferation and migration of breast cancer cells. The low expression of miR-5047 in breast cancer cells can inhibit the proliferation and migration of breast cancer cells. DAC treatment can enhance the expression of miR-5047 in breast cancer cells and induce epithelial transformation of the cells.