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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2020, Vol. 40 Issue (8): 49-54    DOI: 10.13523/j.cb.2005020
综述     
猪多杀性巴氏杆菌检测技术研究进展 *
贾小梅,倪莉,罗洪艳,丁红雷,王豪举()
西南大学动物科技学院 重庆 400715
Research Progress in Pasteurella Multocida Detection Technology
JIA Xiao-mei,NI Li,LUO Hong-yan,DING Hong-lei,WANG Hao-ju()
Laboratory of Veterinary Lemology, College of Animal Science and Technology, Southwest University, Chongqing 400715, China
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摘要：

猪多杀性巴氏杆菌(Pasteurella multocida,Pm)是导致猪呼吸系统疾病的一类重要病原菌,给世界养猪业带来了巨大的经济损失。准确、敏感、快速的Pm检测方法有助于在临床上了解Pm的流行情况,从而采取相应的预防、治疗和综合防控措施。对Pm的病原学、分子生物学、免疫学及分子分型方法的研究现状、原理和优缺点等进行综述,为进一步建立Pm标准检测方法提供参考。
关键词： 猪多杀性巴氏杆菌;  病原学;  分子生物学检测;  分子分型    
Abstract:

Pasteurella multocida (Pm) is an important pathogen that causes respiratory diseases in pigs, which has brought huge economic losses to the world pig industry.Accurate, sensitive and rapid Pm detection method is helpful to understand the prevalence of Pm in clinical practice, so as to take corresponding prevention, treatment and comprehensive prevention and control measures.In this paper, the status, principles, advantages and disadvantages of the research on the etiology, molecular biology, immunology and molecular typing methods of Pm are reviewed in order to provide references for the further establishment of the standard detection methods of Pm.
Key words: Pasteurella multocida    Pathogen    Molecular biology    Molecular typing
收稿日期: 2020-05-11 出版日期: 2020-09-10
ZTFLH:  S852.62  
基金资助: * 国家重点研发计划(SQ2018YFD050073)
通讯作者: 王豪举     E-mail: kyc_whj@swu.edu.cn
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贾小梅
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引用本文:

贾小梅,倪莉,罗洪艳,丁红雷,王豪举. 猪多杀性巴氏杆菌检测技术研究进展 *[J]. 中国生物工程杂志, 2020, 40(8): 49-54.

JIA Xiao-mei,NI Li,LUO Hong-yan,DING Hong-lei,WANG Hao-ju. Research Progress in Pasteurella Multocida Detection Technology. China Biotechnology, 2020, 40(8): 49-54.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.2005020    或    https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I8/49

技术类型 目的基因 检测下限 参考文献
普通PCR kmt1 未提到 [17]
plpE 5个拷贝数 [18]
多重PCR kmt1、toxA、fla 1~10 pg DNA [19]
VFs靶蛋白 102 CFU/反应 [20]
荧光定量PCR toxA 1个基因拷贝 [21]
SodA 10个基因拷贝 [22]
LAMP kmt1 10 CFU/反应 [24]
PlpB 25 CFU/反应 [25]
基因芯片 psl、kmt1 5 pg DNA [27]
表1  猪多杀性巴氏杆菌各项分子生物学检测技术比较
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