中国生物工程杂志

Background: Fusarium graminearum is the major pathogen of Fusarium head blight of wheat and other grain crops. To understand mechnism of pathogenesis of the pathogen, molecular analysis has widely carried out during the past dacades. The differential gene expression (DGE) analysis for MGV1 deletion mutant revealed that the MDT1 gene was significantly down-regulated in MGV1 knock-out mutant, suggesting that the gene was involved in the MGV1 pathway. Go annotation given out that the MDT1 gene was involved in the ATP binding and protein dimerization activity. Objective: In order to identify the function of gene, MDT1(MGV1 dependent transcripts)Gene was isolated and characterized. Methods: The split-marker strategy was applied to construct the deletion cassette of the gene and the phenotype and pathogenicity of the deletion mutant were assayed by conventional method. Results: MDT1 gene deletion mutant significantly decreased the amount of conidia and produced less perithecia than that of the wild type, indicating that the gene was essential for the asexual and sexual reproduction. Vegetative growth of the mutant greatly reduced in solid medium. The mutant also was hypersensitive to cell wall degrading enzyme and formed swollen top of the mycelium and fractured hyphea at 32℃in liquid medium, revealing that the gene was related to the cell wall integration in Fusarium graminearum. The complement test confirmed the phenotypic changes of the MDT1 gene deletion. Conclusion: The results demonstrated that MDT1 gene in Fusarium graminearum was involved in conidiation and vegetative growth.