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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2020, Vol. 40 Issue (5): 105-116    DOI: 10.13523/j.cb.1912023
综述     
生物表面活性剂脂肽的发酵生产及抑菌应用研究进展*
梅雨薇,杨子云,于樊,龙旭伟()
南京理工大学 南京 210094
Recent Progress on Fermentation and Antibacterial Applications of Surfactin
MEI Yu-wei,YANG Zi-yun,YU Fan,LONG Xu-wei()
Nanjing University of Science and Technology, Nanjing 210094,China
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摘要：

表面活性素(surfactin)是一种环脂肽型生物表面活性剂,具有卓越的表/界面活性,能够显著降低水的表面张力,表现出良好的抗真菌、抗病毒、抗肿瘤、杀虫和抗支原体等生物活性,在医药、农业、食品、日化、石油开采等领域具有很大的应用潜力,但高昂的成本和缺乏竞争力的应用领域使其难以真正地实际应用起来。多年来,大量的研究工作在于促进其工业化应用。综述了surfactin的结构、特性及发酵生产,同时系统的比较和总结了surfactin在抑菌方面的应用研究。
关键词： 表面活性素;  芽孢杆菌;  生物表面活性剂;  抑菌    
Abstract:

Surfactin is a kind of cyclic lipopeptide biosurfactant mainly synthesized by many Bacillus subtilis strains. Surfactin is well-accepted as one of the most powerful biosurfactants, presents excellent surface activities, can significantly reduce the surface tension force of water to below 27mN/m at a concentration of 10-30mg/L. In addition, surfactin has great biological activities like: anti-fungal, anti-virus, anti-tumor, insecticidal and anti-mycoplasma, and thus has of great applications potential in the fields of medicine, agriculture, food, cosmetics and petroleum industries. However, its real industrial applications were limited by the high production cost and lack of specific application yields. Many efforts have been done towards the improvement of surfactin production. The recent progresses on surfactin production, and its applications as an antibacterial agent have been reviewed.
Key words: Surfactin    Bacillus    Biosurfactant    Antibacterial
收稿日期: 2019-12-14 出版日期: 2020-06-02
ZTFLH:  Q815  
基金资助: * 国家自然科学基金(51778293)
通讯作者: 龙旭伟     E-mail: xuweilong@njust.edu.cn
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梅雨薇
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引用本文:

梅雨薇,杨子云,于樊,龙旭伟. 生物表面活性剂脂肽的发酵生产及抑菌应用研究进展*[J]. 中国生物工程杂志, 2020, 40(5): 105-116.

MEI Yu-wei,YANG Zi-yun,YU Fan,LONG Xu-wei. Recent Progress on Fermentation and Antibacterial Applications of Surfactin. China Biotechnology, 2020, 40(5): 105-116.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.1912023    或    https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I5/105

图1  Surfactin的化学结构
Microorganism Scale Surfactin yield(g/L) Reference
B. subtilis ATCC 21332 500ml flask 0.33 [22]
B. amyloliquefaciens MT45 500ml flask 1.04 [23]
B. subtilis CWS1 500ml flask 0.99 [24]
Bacillus subtilis MZ-7 250ml flask 0.28 [25]
Bacillus subtilis BS5 250ml flask 0.3 [26]
Bacillus subtilis E8 3.7L 0.75 [27]
B. subtilis SPB1 250ml flask 0.72 [28]
Microorganism Surfactin yield (g/L) Strain modifications Surfactin yield(g/L) Reference
B. subtilis fmbR 0.38 Replacement of PsrfA with Pspac 3.86 [29]
B. subtilis THY-7 0.55 Replacement of PsrfA with PgroE 0 [30]
Replacement of PsrfA with PsacB 1.09
Replacement of PsrfA with PsacP 0.22
Replacement of PsrfA with Pg1 1.44
Replacement of PsrfA with Pg2 5.98
Replacement of PsrfA with Pg3 9.74
B. subtilis THY-15 1.2 Replacement of PsrfA and Pg3 8.2 [31]
Introduce VHb gene 10.2
B. subtilis 168 0 Introduce srfA gene 0.4 [32]
Deleting the biofilm formation-related genes 1.4
Deleting the nonribosomal peptide synthetase/polyketide
synthase pathways		 1.7
Over-expressing potential self-resistance associated proteins 3.8
Engineering the branched-chain fatty acid biosynthesis pathway 8.5
Enhancing srfA transcription 12.8
表1  不同菌株生产surfactin的产量
Microorganism Agro-industrial waste Addition of agro-industrial waste Surfactin yield Reference
Bacillus amyloliquefaciens XZ-173 Rice straw and soybean flour 5g soybean flour, 4g rice straw, 2%
maltose and 2.65% glycerol		 15.03mg/gds [41]
Bacillus subtilis MTCC 2423 Rice mill polishing residue Total carbohydrate : 4.76g/L 4.17g/kg residue [42]
Bacillus subtilis MTCC 2423 Waste frying sunflower oil 50g/L 14.9mg/g [43]
Waste frying rice bran oil 50g/L 11mg/g
Bacillus subtilis Hydrolysis of olive mill waste 5% 26.5mg/L [44]
B. amyloliquefaciens MT45
and B. amyloliquefaciens X82		 Distillers' grains 200g/L 3.4g/L [23]
Bacillus subtilis 37 Feather hydrolysate waste
and glutamate mill waste		 FHW (1%, V/V)
GMW (4%, V/V),		 0.523g/L [45]
Bacillus pumilus UFPEDA 448 Okara 50% 3.3g/kg dry solids [46]
Bacillus subtilis ATCC 6633 Rehydrated whey powder 10%~20% 0.18~0.29g/L [47]
表2  不同芽孢杆菌利用农工业废渣生产surfactin的产量
图2  Surfactin 破坏细胞膜机制图
Microorganism Inhibited-strain MIC(g/L) MBC(g/L) Reference
B. subtilis MSH1 Shigella dysenteriae
Staphylococcus aureus		 0.15
0.2		 0.2
0.25		 [72]
Bacillus subtilis S. aureus ATCC65389 0.032 0.128 [69]
B. subtilis MB198, MB245, MB199,
and MB200		 Candida albicans SC5314 >0.1 [39]
Bacillus safensis F4 Staphylococcus epidermidis S61
Staphylococcus aureus ATCC 9144
Agrobacterium tumefaciens		 6.25
1.56
3.125		 [73]
B. subtilis AM1 Legionella bozemanii ATCC 33217 0.001 [74]
B. subtilis PB2-L1 Fusarium moniliforme ATCC3893 0.05 [75]
Bacillus subtilis ATCC 6633 Staphylococcus aureus ATCC 29213 >0.5 [76]
Bacillus subtilis pB2 Staphylococcus aureus AS1.2465 0.02 [77]
表3  Surfactin对不同菌株的MIC 和MBC值
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