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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2020, Vol. 40 Issue (5): 78-83    DOI: 10.13523/j.cb.1912002
综述     
噬菌体分离纯化技术研究进展*
秦旭颖,杨洪江()
工业发酵微生物教育部重点实验室 天津市工业微生物重点实验室 天津科技大学生物工程学院 天津 300457
Research Progress on Techniques for Separation, Purification of Bacteriophages
QIN Xu-ying,YANG Hong-jiang()
Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
 全文: PDF(523 KB)   HTML
摘要：

噬菌体应用领域十分广泛,因此在制备噬菌体过程中,需采用不同的技术或几种技术相结合的方法来获得具有不同纯度的噬菌体制剂。常用的技术主要包括沉淀、过滤和离心。近年来,色谱技术、场流分流技术和电泳技术等的应用,为制备噬菌体制剂提供了新的方向。
关键词： 噬菌体;  色谱;  场流分馏;  介电电泳    
Abstract:

Bacteriophage is widely used in many fields, so in the process of preparing phage, the different techniques or several techniques to obtain phage preparations with different purity to be used. The techniques commonly used to separate and purify phage are precipitation, filtration and centrifugation. In recent years, the application of chromatographic, field-flow fractionation techniques and electrophoresis techniques provides a new direction for obtaining phage preparations.
Key words: Bacteriophage    Chromatography    Field-flow fractionation    Dielectrophoresis
收稿日期: 2019-12-01 出版日期: 2020-06-02
ZTFLH:  Q815  
基金资助: * 国家自然科学基金(31970150)
通讯作者: 杨洪江     E-mail: hongjiangyang@tust.edu.cn
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引用本文:

秦旭颖,杨洪江. 噬菌体分离纯化技术研究进展*[J]. 中国生物工程杂志, 2020, 40(5): 78-83.

QIN Xu-ying,YANG Hong-jiang. Research Progress on Techniques for Separation, Purification of Bacteriophages. China Biotechnology, 2020, 40(5): 78-83.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.1912002    或    https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I5/78

图1  非对称流场流分馏技术原理示意图
图2  基于绝缘体的介电电泳通道示意图
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