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| Isolation, Identification and Characterization of a Biofilm Formation Strain |
| LI Long-jie1,2, ZHOU Gang2, SHI Qing-shan2, OUYANG You-sheng2, CHEN Yi-ben2, HU Wen-feng1 |
1. College of Food Science, South China Agricultural University, Guangzhou 510642, China;
2. Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangzhou 510070, China |
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Abstract Objective: Isolate and screen a strain that has the ability to form biofilms from the samples of industrial spoilage with isothiazolinone, meanwhile research its preliminary characterization of biofilm formation. Methods:The streak plate and crystal violet staining methods were used to isolate and screen the biofilm-forming strain respectively. Morphological, physiological characteristics and 16S rDNA sequence analysis were adopted to determine the phylogenetic position of the isolated strain. The biofilms formed on the glass surface were observed under confocal scanning laser microscopy, including exopolysaccharides and live or dead cells. The influence of culture time, pH and different carbon sources on biofilm formation was also researched by crystal violet staining method in the microtiter plates. Results: A biofilm-forming strain was successfully isolated and named as BF-17, which was identified and clarified into Enterobacter cloacae. The highest biofilm biomass was found at the time of 96 h or pH 5.0, respectively. After static cultured for 4 days, biofilms of BF-17 formed on the glass surface exhibited a representative morphological characteristics and structures of the typical biofilms. Meanwhile, the highest biofilm formation was formed in the M9 medium with the supplementation of α-lactose or D-fructose respectively. Whereas, addition of citric acid in M9 medium made the lowest biofilm formation. Conclusion: E. cloacae BF-17 shows a stable capacity of biofilm formation and can be used as a sample for further biofilm study. Moreover, the biofilm formation of BF-17 can be influenced by various external environment factors.
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Received: 22 August 2013
Published: 25 November 2013
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