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| The Effect of Carbon and Nitrogen Sources on Laccase Production and Properties from Fomes fomentarius |
| SHANG Jie1,2, WU Qiu-xia2, LIAN Xiao-long2, WANG Qiu-yu1 |
1. Northeast Forestry University, College of Life Science, Harbin 150040, China;
2. Beifang University of Nationalities, College of Biological Science and Engineering, Yinchuan 750021, China |
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Abstract The production of laccase by Fomes fomentarius was studied. Cultures conditions involving variations in carbon and nitrogen sources and different C:N ratios were examined at constant temperature and pH, with the aim of increasing yield of laccase. And its enzymatic properties were determined. The best results were obtained when using wheat bran and peptone as carbon and nitrogen sources respectively with a C:N ratio of 10.4. Compared to the initial medium, the highest laccase yield observed is approximately increased by 7.88 times under the optimized conditions. The optimum pH and temperature for its activity is 3.0 and 50℃ with the corresponding Km and Vmax of 0.20 mmol/L and 2.58 mmol/L·min respectively. DTT(0.1mmol/L) and NaN3(10mmol/L) nearly inhibit all activity of the laccase, as well as the metal ions especially Ba2+, Ca2+, Co2+ and Fe2+(10mmol/L). In summary, our results will provide basic information to the production of laccase by Fomes fomentarius and the utilization of environmental engineering in the future.
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Received: 14 June 2013
Published: 25 November 2013
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