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中国生物工程杂志

China Biotechnology
China Biotechnology  2010, Vol. 30 Issue (05): 49-56    DOI:
    
A Set of Genes Up-regulated by Cadmium Ions and Their Function under Cadmium Stress in Arabidopsis thaliana
1.School of Life Science, East China Normal University, Shanghai 200062, China
2.College of Life Science and Chemistry, Xinjiang Normal University, Urumqi 830054, China
3.Shanghai Information Center for Life Science,CAS, Shanghai 200031 China
4.College of Life Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China
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Abstract  

Cadmium is a kind of highly toxic heavy metals. Even very low concentration of Cd2+ in soil solutions can result in toxic effects to plants. To survive, plants must change their metabolism to cope with cadmium exposure. Before that a lot of genes had changed their expression. A novel differential display PCR method was adopted that is based on annealing control primers (ACPs) to identify up-regulated genes of Arabidopsis by Cd2+ exposure. Nineteen differentially expressed bands were isolated and sequenced. They represent eighteen genes. Among them, six genes were identified by RT-PCR that they were really induced by cadmium treatment, including LEA(late embryogenesis abundant protein), AtGSTF2 (Glutathione S-transferase 2), AtGSTF6(Glutathione S-transferase 6), HSP70(heat shock protein 70), sHSP17.6B-CI(17.6 kDa class I small heat shock protein) and sHSP17.6-CII(17.6 kDa class II small heat shock protein). The results will help us to understand detoxification mechanism of plant to cadmium. And promoters of these three HSPs could be used for phytoremediation of cadmium pollution.



Key wordsACPCadmium exposure      Late embryogenesis abundant protein      Heat shock protein      Glutathione S-transferase     
Received: 20 January 2010      Published: 25 May 2010
Fund:  

Project supported by National Natural Science Foundertion(30660014),SRF for ROCS, SEM(2006), the Natural Scientific Foundation of Inner Mongolia(200508010503)

Cite this article:

CENG Wei-Jun, WANG Shui-Beng, LI Xiao-Fang, XU Ping, WANG Rui-Gang. A Set of Genes Up-regulated by Cadmium Ions and Their Function under Cadmium Stress in Arabidopsis thaliana. China Biotechnology, 2010, 30(05): 49-56.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2010/V30/I05/49

[1] Sanità di Toppi L, Gabbrielli R.Response to cadmium in higher plants. Environmental and Experimental Botany, 1999,41(2):105130. 
[2] Fusco N, Micheletto L, Giovanni D C, et al. Identification of cadmiumregulated genes by cDNAAFLP in the heavy metal accumulator Brassica juncea L.Journal of Experimental Botany, 2005,56(421): 30173027. 
[3] Vassilev A, Tsonev T, Yordanov I.Physiological response of barley plants (Hordeum vulgare) to cadmium contamination in soil during ontogenesis. Environ Pollut, 1998,103:287293. 
[4] Lang M L, Zhang Y X, Chai T Y. Identification of genes upregulated in response to Cd exposure in Brassica juncea L. Gene, 2005,363:151158. 
[5] Sullivan J A, Shirasu K, Deng X W. The diverse roles of ubiquitin and the 26S proteasome in the life of plants. Nat Rev Genet, 2003,4(12): 948958. 
[6] Suzuki N, Koizumi N, Sano H. Screening of cadmium responsive genes in Arabidopsis thaliana. Plant, Cell and Environment, 2001,24(11): 11771188. 
[7] Tocquin P, Corbesier L, Havelange A, et al. A novel high efficiency, low maintenance, hydroponic system for synchronous growth and flowering of Arabidopsis thaliana. BMC Plant Biology, 2003,3: 2. 
[8] Kim Y J, Kwak C I, Gu Y Y, et al. Annealing control primer system for identification of differentially expressed genes on agarose gels. BioTechniques, 2004, 36(3):424426. 
[9] Cui X, Shin M, Lee K,et al. Identification of differentially expressed genes in murine embryos at the blastocyst stage using annealing control primer system. Molecular Reproduction And Development, 2005,70(3):278287. 
[10] Shao H B, Liang Z S, Shao M G. LEA proteins in higher plants: Structure, function, gene expression and regulation.Colloids and Surfaces B: Biointerfaces, 2005, 45(34):131135. 
[11] Singh S, Cornilescu C C, Tyler R C, et al. Solution structure of a late embryogenesis abundant protein(LEA14) from Arabidopsis thaliana, a cellular stressrelated protein. Protein Science, 2005, 14(10):26012609. 
[12] Goyal K, Walton L J, Tunnacliffe A. LEA proteins prevent protein aggregation due to water stress. Biochem J,2005, 388, 151157. 
[13] Krüger C, Berkowitz O, Stephan U W, et al. A Metalbinding member of the late embryogenesis abundant protein Family transports iron in the phloem of Ricinus communis. The Journal of Biological Chemistry, 2002,277(28): 2506225069. 
[14] Smith A P, DeRidder B P, Guo W J, et al. Proteomic analysis of Arabidopsis glutathione Stransferases from benoxacor and coppertreated seedlings. The Journal of Biological Chemistry, 2003, 279(25): 2609826104. 
[15] Smith A P, Nourizadeh S D, Peer W A, et al. Arabidopsis AtGSTF2 is regulated by ethylene and auxin, and encodes a glutathione Stransferase that interacts with flaonoids. The Plant Journal, 2003,36(4):433442. 
[16] Lieberherr D, Wagner U, Dubuis P H, et al. The rapid induction of glutathione STransferases AtGSTF2 and AtGSTF6 by avirulent Pseudomonas syringae is the result of combined salicylic acid and ethylene signaling. Plant Cell Physiol,2003, 44(7):750757. 
[17] Hamiltone W, Coleman J S. Heatshock proteins are induced in unstressed leaves of Nicotiana attenuata (Solanaceae) when distant leaves are stressed. American Journal of Botany, 2001, 88(5):950955. 
[18] Sun W N, Bernard C, Cotte B,et al. AtHSP17.6A, encoding a small heatshock protein in Arabidopsis, can enhance osmotolerance upon overexpression. The Plant Journal, 2001,27(5):407415. 
[19] Waters E R, Lee G J, Vierling E. Evolution, structure and function of the small heat shock proteins in plants. J Exp Bot,1996,47(296): 325338. 
[20] Ehrnsperger M, Graber S, Gaestel M, et al. Binding of nonnative protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation. EMBO J,1997,16(2): 221229. 
[21] Lee G J, Roseman A M, Saibil H R, et al. A small heat shock protein stably binds heatdenatured model substrates and can maintain a substrate in a foldingcompetent state. EMBO J,1997, 16(3): 659671. 
[22] Lee G J, Vierling E A. Small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heatdenatured protein. Plant Physiology, 2000,122(1): 189197. 
[23] K renlampi S, Schat H,Vangronsveld,et al. Genetic engineering in the improvement of plants for phytoremediation of metal polluted soils. Environmental Pollution, 2000, 107(2):225231. 
[24] Tawa V S, Dinkins R D, Palli S R, et al. Development of a methoxyfenozideresponsive gene switch for applications in plants. The Plant Journal,2006, 45(3):457469.

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