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| Molecular Biology Identification of ANAMMOX Bacteria in Single-stage Autotrophic Nitrogen Removal System |
| HUANG Jun-li1,ZOU Han-yan1,WANG Gui-xue1,FANG Fang2,GUO Jing-song2 |
1.College of Biological Engineering Chongqing University, Chongqing 400030, China
2.Key Laboratory of the Three Gorges Reservoir Region’s EcoEnvironment, Ministry of Education,Chongqing University, Chongqing 400045, China |
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Abstract A deeper analysis of the role of anaerobic ammonium oxidation (ANAMMOX) bacteria will help to understand its application in the biological nitrogen removal. In this study, using the single-stage autotrophic nitrogen removal system with stable operation and 90% of ammonia conversion rate and 80% of total nitrogen removal rate, the total DNA of cultivated sludge taken from the bottom of the system was extracted by molecular biology methods. With the total DNA partial 16S rDNA sequence of ANAMMOX bacteria was amplified by polymerase chain reaction (PCR) with a pair of specific primers Pla46rc/Amx820. Amplified product was cloned, sequenced and BLAST analysis and the result indicated that the sequence has 99% identities with ANAMMOX bacteria Candidatus Kuenenia stuttgartiensis and Candidatus Brocadia anammoxidans. The phylogenetic analysis showed that the anaerobic ammonium-oxidizing bacteria in the single-stage autotrophic nitrogen removal system have closer relationship with Candidatus Kuenenia stuttgartiensis evolutionarily.
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Received: 30 December 2009
Published: 12 June 2010
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Corresponding Authors:
huang junli
E-mail: huang_junli@126.com
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