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中国生物工程杂志

China Biotechnology
China Biotechnology  2023, Vol. 43 Issue (4): 123-132    DOI: 10.13523/j.cb.2209048
    
Research Progress of Alcohol Production by Cell Catalytic Technology
ZHANG Yan,LIU Xiu-dong()
College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China
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Abstract  

As an important part of biocatalysis, whole-cell catalysis uses whole cells as catalysts, which eliminates the need of cell lysis and enzyme purification in the process of alcohol production, and thus greatly reduces the cost of alcohol production. At the same time, the cell wall components can protect the trapped enzyme from potentially harmful environmental factors, thus meeting the production requirements of low cost and high stability of catalysts. According to the advantages of cell catalysis technology and the current market application prospects, the suitable strains for producing alcohol substances, such as ethanol, diol and sugar alcohol, were discussed. The alcohol production by using cell catalysis technology in food and chemical industries was reviewed. Finally, the production strategy of increasing alcohol yield was also discussed.



Key wordsBiocatalysis      Cell catalysis      Ethanol      Glycol      Sugar alcohol     
Received: 17 September 2022      Published: 04 May 2023
ZTFLH:  Q819  
Cite this article:

ZHANG Yan, LIU Xiu-dong. Research Progress of Alcohol Production by Cell Catalytic Technology. China Biotechnology, 2023, 43(4): 123-132.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2209048     OR     https://manu60.magtech.com.cn/biotech/Y2023/V43/I4/123

产物 菌株 底物 产量/
(g·L-1)
得率
(g/g)
生产规模 参考文献
乙醇 酿酒酵母(S. cerevisiae WXY12) 葡萄糖和木糖 46.87 0.15 摇瓶 [8]
运动发酵单胞菌(DSM 473) 葡萄糖 47.93 0.475 摇瓶 [9]
产气大肠杆菌(TISTR 1468) 甘油和乙酸 32.1 0.125 100 mL生物反应器 [10]
酿酒酵母(S. cerevisiae) 玉米秸秆汁液 62.12 0.45 摇瓶 [11]
酿酒酵母(S. cerevisiae) 龙舌兰 (Agave tequilana)叶(一种农业残留物) 12.2±0.3 0.41 摇瓶 [12]
大肠杆菌(Escherichia coli SL100) 大麦秸秆 19.43 0.16 4.5 L生物反应器 [13]
假丝酵母(JCM 1644) 木质纤维素 55.64 0.45 摇瓶 [14]
2-苯乙醇 酿酒酵母(S. cerevisiae YS58) L-苯丙氨酸 6.3 0.7 5 L生物反应器 [15]
酿酒酵母(S. cerevisiae YS58) L-苯丙氨酸 3.73 0.68 摇瓶 [16]
酿酒酵母(S. cerevisiae MT2) L-苯丙氨酸 3.59 0.72 摇瓶 [17]
大肠杆菌(E. coli BW25113) L-苯丙氨酸 5.32 0.65 200 μL全细胞催化生物反应器 [18]
地衣芽孢杆菌(Bacillus licheniformis DW2) L-苯丙氨酸 5.16 0.65 摇瓶 [19]
酿酒酵母(S. cerevisiae zzuli-1) 烟草废料提取物 1.65 0.34 摇瓶 [20]
马克斯克鲁维酵母(Kluyveromyces marxianus) 甜乳清 1.2 0.39 摇瓶 [21]
1, 3-
丙二醇
肺炎克雷伯氏菌(Klebsiella pneumoniae) 葡萄糖和戊糖 20.88 0.69 摇瓶 [22]
肺炎克雷伯氏菌(K. pneumoniae) 葡萄糖和粗甘油 29.69 0.84 5 L生物反应器 [23]
丁酸梭菌(DSM 4278) 粗甘油 12.69±
0.9
0.35±
0.02
1.3 L生物反应器 [24]
罗伊氏乳杆菌(Lactobacillus reuteri CH53) 葡萄糖和甘油 68.32±
0.84
0.83±
0.02
5 L生物反应器 [25]
谷氨酸棒杆菌(Corynebacterium glutamicum) 葡萄糖和木糖 110.4 0.42 5 L生物反应器 [26]
酿酒酵母(S. cerevisiae) 甘油 3.58 0.15 摇瓶 [27]
2, 3-
丁二醇
肺炎克雷伯氏菌(K. pneumoniae) 葡萄糖 91 0.45 摇瓶 [28]
巴西类芽孢杆菌(Paenibacillus brasilensis PB24) 葡萄糖 27 0.43 摇瓶 [29]
路德维希肠杆菌(Enterobacter ludwigii) 蔗糖和甘蔗糖蜜 50.6 0.31 6.7 L生物反应器 [30]
地衣芽孢杆菌(B. licheniformis YNP5-TSU) 葡萄糖 99.3 0.41 1 L生物反应器 [31]
酿酒酵母(S. cerevisiae BY4741) 红海藻(Gmandium amansii)水解产物 14.8 0.3 50 mL不锈钢间歇式反应器 [32]
路德维希肠杆菌(E. ludwigii BW) 葡萄糖和富含葡萄糖的水解产物 144.5 0.47 2.5 L生物反应器 [33]
Table 1 Summary of alcohol production with microbes
多元醇 微生物生产 底物 产量/(g·L-1) 生产规模 参考文献
赤藓糖醇 解脂耶氏酵母 甘油 81.9 5 L生物反应器 [39]
真菌花粉念珠菌(Moniliella pollinis) 甘蔗汁和糖蜜 94.9 5 L生物反应器 [40]
嗜渗透酵母(Moniliella sp. BCC25224) 葡萄糖 138 10 L生物反应器 [41]
甘露糖醇 念珠菌(SK 26.001) 葡萄糖 97.1 30 L生物反应器 [36]
青霉菌(Penicillium sp. T2-M10) 葡萄糖 1.26 摇瓶 [37]
假肠系膜明串珠菌(CTCC G123) 葡萄糖 88.1 3 L生物反应器 [42]
山梨糖醇 运动发酵单胞菌(ZM4) 蔗糖 45.62±2.7 2 L生物反应器 [43]
耐热运动发酵单胞菌(TISTR548) 甘蔗渣和木薯浆水解产物 5.89 摇瓶 [44]
木糖醇 假单胞菌(BSX-46) 木糖 35.2 3 L生物反应器 [45]
酿酒酵母(S. cerevisiae) 木糖母液和玉米芯残渣 91 1.4 L生物反应器 [46]
工程化酿酒酵母 木糖 12.4 摇瓶 [47]
Table 2 Summary of sugar alcohol production with microbes catalysis
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