Research Progress on DNA Double-Strand Break Assay

doi:10.13523/j.cb.2005013

China Biotechnology

2020, Vol. 40

Issue (8): 55-62 DOI: 10.13523/j.cb.2005013

Research Progress on DNA Double-Strand Break Assay

DAI Han-ying,XU Ke-qian(

)

Department of Laboratory Medicine, Xiangya School of Medicine, Central South University, Changsha 410013, China

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Abstract

Different factors affect DNA integrity, which can be divided into endogenous and exogenous factors, these factors can cause different degrees of DNA damage. Among them, DNA double-strand break is the most serious kind of DNA damage, which is characterized by two DNA strands being cut off. If it is not repaired in time, it will cause a series of damage reactions. Serious DNA double strand break can even cause severe consequences such as cell apoptosis and tumor. Therefore, rapid and accurate detection of the degree of DNA double-strand break in cells is helpful to assess DNA integrity, the effects of genetic toxicity in and out of the environment, clinical diagnosis and chemoradiotherapy monitoring. DNA double-strand break detection technology has developed rapidly in recent years. According to the principle, it can be roughly divided into physical or chemical methods, immunofluorescence method and high-throughput sequencing method. These methods detect the physical and chemical characteristics of the products after DNA double-strand break or specific molecular markers, so as to evaluate the degree of DNA double-strand break. The latest research progress, application and advantages and disadvantages of these methods were introduced in order to provide guidance for the future research and clinic of DNA double-strand break detection.

Key words： DNA double-strand break assay Comet assay γ-H2AX High-throughput sequencing

Received: 08 May 2020 Published: 10 September 2020

ZTFLH:

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Corresponding Authors: Ke-qian XU E-mail: xukeqian@csu.edu.cn

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Cite this article:

DAI Han-ying,XU Ke-qian. Research Progress on DNA Double-Strand Break Assay. China Biotechnology, 2020, 40(8): 55-62.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2005013 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I8/55

Fig.1 The schematic of DSBs detection by Comet assay

Fig.2 LM-PCR schematic

Fig.3 The schematics of DSBs detection by BLESS and BLISS (a) The schematics of BLESS (b) The schematics of BLISS

Table 1 Comparison of different DSBs high-throughput sequencing techniques

Table 2 Methodology evaluation of major DSBs detection technologies


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