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| The Investigation of RNA Viruses in Mosquitoes via High-Throughput Sequencing |
| WANG Hui-yu1, HE Tong-tong2, LIU Jia-jia1, FAN Hang2, SONG Feng-lin3, MEI Lin1, TONG Yi-gang2, HAN Xue-qing1 |
1. Chinese Academy of Inspection and Quarantine, Beijing 100176, China;
2. Beijing Institutes of Microbiology and Epidemiology, Beijing 100071, China;
3. Liaoning Entry-Exit Inspections and Quarantine Bureau, Dalian 116001, China |
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Abstract Objective:Based on high-throughput sequencing, the diversity of mosquito-borne viruses in different border areas of China was analyzed, which provided information for rapid screening of mosquito-borne virus. Methods:Field samples from different border areas were randomly divided into 6 groups and virus nucleic acids were extracted, followed byhigh-throughput sequencing. After that, bioinformatics methods were used to analyze the sequencing reads as well as the status of virus infection in themosquitoes'sample.Results:In the six samples, four mixed samples were found to containtwo or more suspicious viruses.Conclusion:High throughput sequencing detection system can effectively detect the presence of viruses, and preliminarily revealed the diversity of viruses carried by mosquitoes.
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Received: 26 September 2017
Published: 15 November 2017
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