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| The Role of CodY in the Regulation of Flagellar Motility and Virulence in Listeria monocytogenes |
| ZHANG Ying, TANG Yu-qian, SHEN Yi, LUO Qin |
| Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China |
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Abstract Objective: To explore the function of the transcription factor CodY on flagellar motility, bacterial virulencein Listeria monocytogenes. Methods: The codY gene on the L.monocytogenes chromosome was knocked out by homologous recombination and the strain of the deleted codY gene was successfully complemented by the codY gene construct;the results were evaluated by observation of the motilityof flagellar movement, the transcriptional expression of flagellum-related genes with RT-qPCR.The effects of wild-type strain EGDe and CodY-deficient strain on bacterial hemolytic activity, semi-lethal dose of Helicoverpa armigera and the main virulence factor LLO(coding gene hly) and virulence regulatory protein PrfA(coding gene prfA) transcription expression profiles were compared.Results: Compared with the wild-type strain, the CodY-deficient strain showed a significant reduction in the flagellar movement, the hemolytic activity, as well as the transcriptional expression of flagellum-related genes and the major virulence genes hly and prfA(P ≤ 0.01); while the semi-lethal dose on Helicoverpa armigera (Hübner) was increased 5.8 fold.Conclusion: The CodY plays an important role in flagellar motility and transcription regulation ofbacterial virulence in Listeria monocytogenes.
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Received: 13 February 2017
Published: 25 July 2017
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