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| Human Amnion Mesenchymal Stem Cells Positioned Transplantation for the Treatment of Mice Liver Damage Induced by Carbon Tetrachloride |
| CONG Shan1, WANG Xiu-mei1, LI Yan1, SONG Jing1, BAI Li-heng2, CAO Gui-fang1 |
1. Department of Veterinary Laboratory of Animal Embryology and Developmental Biology, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Department of Maternity, Inner Mongolia Maternal and Child Health Hospital, Hohhot 010020, China |
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Abstract Objective: To observe the therapeutic effect of human amnion mesenchymal stem cells transplantation labeled with 5, 6, 2-carboxy fluorescein diacetate succinimidy ester(CFSE)on mice model with liver damage induced by carbon tetrachloride. Methods: The amnion was mechanically separated from the placenta obtained by cesarean section after 38~40 week-pregnancy. The amniotic mesenchymal stem cells were obtained by using two-step digestion with trypsin and collagenase type I, then the flow cytometry and immunofluorescent staining techniques were adopted to identify the surface molecular markers and stem cell characteristics. Hepatic damage model groups were established through carbon tetrachloride and oil inducing, 20 μl/g doses of the mixture were given to mice by abdominal injection. Mouse models of hepatic damage were randomly divided into two groups. The control group was injected with the same volume of PBS via the tail vein; the treatment group was injected with human amnion mesenchymal stem cells suspension labeled with CFSE, and the number of the stem cells was 1×106. At 4 weeks after transplantation, animals were sacrificed. Liver tissues were obtained for the histological observation and the eyeball blood was collected to detect the liver function indicators. Results: The human amniotic mesenchymal stem cells obtained by using two-step digestion with trypsin and collagenase type I were high-purity. At 1 weeks after transplantation, the immunofluorescent staining of frozen slice showed that human amnion mesenchymal stem cell colonization could be seen in the mice liver tissues of the cell transplantation group. The labeled human amnion mesenchymal stem cell showed strong green fluorescence. At 4 weeks after cell transplantation, compared with the model group, levels of serum aspartate aminotransferase, alanine aminotransferase in the cell transplantation group were significantly decreased, while the total albumin level was increased significantly. The liver cell inflammatory necrosis, steatosis and liver fibrosis were improved significantly. After 4 weeks, the immunofluorescent staining of frozen slice result indicated that the expressions of human serum albumin could be observed in the mice liver tissue of the cell transplantation group, but no expression could be seen in the model group. It is visible that human amnion mesenchymal stem cells can improve liver function and pathological damage of liver damage mice in a certain extent. The findings may provide useful experimental datas for cell positioned transplantation with treatment liver disease.
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Received: 03 June 2014
Published: 25 August 2014
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