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| The Optimization Research of Fermentation Medium of γ-Polyglutamic Acid(γ-PGA) Produced by Bacillus natto |
| ZHANG Wen1,2, ZHANG Shu-qing1,2, MA Xiao-tong1, HE Cui-cui1 |
1. Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China;
2. Plant Nutrition Laboratory, Ministry of Agriculture, Beijing 100125, China |
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Abstract Optimization of fermentation medium for Bacillus natto ZW-2 to produce γ-polyglutamic acid(γ-PGA)was studied to increase the yield of γ-polyglutamic with the shaking-flask culture. With Design-Export software, Plackett-Burman Design was used to evaluate the effect of the factors on Bacillus natto ZW-2 to produce γ-PGA based on single-factor optimization experiment. Then steepest ascent experiments was designed to approach the maximum production area of γ-PGA.At last, Box-Behnken experiment was used to get the optimal fermentation medium.The results showed that the optimal composition was sodium glutamate 68.76g/L, NH4Cl1.16g/L, K2HPO42.16g/L. On this condition the average yield of the verifying experiment was 36.421g/L, which was 1.54 times the original medium. Plackett-Burman design and Box-Behnken Design were simultaneously used in the optimization of the fermentation of Bacillus natto ZW-2 to produce γ-PGA, which was proved to be effective and economical.
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Received: 17 September 2013
Published: 25 November 2013
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[1] 吕忠良. γ-聚谷氨酸(γ-PGA)的分离纯化研究.杭州:浙江大学材料与化学工程学院, 2008. Lv Z L.Study on separation and purification of poly-γ-glutamic acid from broth.Hangzhou:Zhejiang University, Department of Materials Sciences and Chemical Engineering, 2008.
[2] Meynell G G, Meynell E. The biosynthesis of poly d-glutamic acid, the capsular material of Bacillus anthracis. J GEN Microbiol, 1966.43(1):p.119-138.
[3] Bovarnick M. The formation of extracellular d-glutamic acid polypeptide by Bacillus subtilis, JBiolChem, 1942, 145:415-424.
[4] 游庆红, 张新民, 陈国广, 等.γ-聚谷氨酸的生物合成及应用.现代化工, 2002, 22(12):56-59. You Q H, Zhang X M, Chen G G, et al.Biosyllthesis and appljcation of poly(γ-glutamic acid).Modern Chemical Industry, 2002, 22(12):56-59.
[5] 吴学超, 曹新江, 冀志霞, 等. 聚γ-谷氨酸高产菌的选育与培养基优化.微生物学通报, 2008, 35(10):1527-1531. Wu X C, Cao X J, Yi Z X, etal.Screening of poly-γ-glutamic acid high productive strain and optimization of fermentationmedium.Microbiology, 2008, 35(10):1527-1531.
[6] GEP Box, KB Wilson. On the experimental attainment of optimum conditions.Journal of the Royal Statistical Society, 1951, 13(1):l-45.
[7] 王永菲, 王成国.响应面法的理论与应用.中央民族大学学报(自然科学版), 2005, 14(3):236-240. Wang Y F, Wang C G.The application of response surface methodology.Journal of the CUN(Natural Sciences Edition), 2005, 14(3):236-240.
[8] 张泽志, 韩春亮, 李成未.响应面法在实验设计与优化中的应用.河南教育学院学报(自然科学版), 2011, 20(4):34-37. Zhang Z ZH, Han CH L, Li CH W.Application of response surface method in experimental design and optimization.Journal of Henan Institute of Education(Natural Science Edition), 2011, 20(4):34-37.
[9] Plackett R L, Burman J P. The design of optimum multifactorial experiments, Biometrika, 1946, 33(4):305-325.
[10] 郝学财, 余晓斌, 刘志钰, 等.响应面方法在优化微生物培养基中的应用.食品研究与开发, 2006, 27(1):38-41. Hao X C, Yu X B, Liu ZH Y, et al.The application of response surface methodology in optimization of microbial media.Food Research and Developent, 2006, 27(1):38-41.
[11] Fernanda F, Béal C, Corrieu G. Operating conditions that affect the resistance of lactic acid bacteria to freezing and frozen storage.Cryobiology, 2001, 43(3):189-198.
[12] 张晓萍, 杨静, 勇强, 等.响应面优化法在纤维素酶合成培养基设计上的应用.林产化学与工业, 2010, 30(3):29-34. Zhang X P, Yang J, Yong Q, et al.Statistical optimization of process parameters on cellulase production. Chemistry and Industry of Forest Products, 2010, 30(3):29-34.
[13] 潘春梅, 樊耀亭, 赵攀. 发酵法产氢培养基的响应面分析优化.农业生物技术科学, 2008, 24(1):38-44. Pan CH M, Fan Y T, Zhao P.Statistical optimization of prosess parameters on biohydrogen production from glucose by clostridium sp.Fanp2.Chinese Agricultural Science Bulletin, 2008, 24(1):38-44.
[14] 曹小红, 蔡萍, 李凡, 等.利用响应面法优化Bacillus natto TK-1产脂肽发酵培养基.中国生物工程杂志, 2007, 27(4):59-65. Cao X H, Cai P, Li F, et al.Medium optimization for lipopeptide produced by Bacillus nattoTK-1 using response surface methodology.China Biotechnology, 2007, 27(4):59-65.
[15] 汪彬彬, 车振明. Plackett-Burman和Box-Benhnken Design实验设计法优化华根霉产糖化酶发酵培养基的研究.食品科技, 2011, 36(5):41-45. Wang B B, Che Z M.Optimization of rhizopus fermentation medium for glucoamylase production by Plackett and Box-BenhnkenDesign.Food Science and Technology, 2011, 36(5):41-45.
[16] 张艳丽, 高华, 刘小红.微生物合成的聚谷氨酸及其应用.生物技术通报, 2008, 4:58-62. Zhang Y L, Gao H, Liu X H.Biosynthesis and application of polyglutamicacid.Biotechnology Bulletin, 2008, 4:58-62.
[17] 王勇, 伊玉森, 张远征.无机盐及微量元素对谷氨酸发酵的影响.发酵科技通讯, 2004, 33(3):2-3. Wang Y, Yi Y S, Zhang Y ZH.FAJIAO KEJI TONGXUN, 2004, 33(3):2-3. |
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