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| Increased Co-production of S-adenosylmethionine and Glutathione by Sodium Citrate Addition |
| WANG Yu-lei, ZHU Jian, WEI Gong-yuan, XU Hong-qing, WANG Cheng-fu |
| School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China |
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Abstract S-adenosylmethionine (SAM) and glutathione (GSH) are both important small S-contained compounds in cells. The effects of sodium citrate on the fermentative co-production of SAM and GSH with Candida utilis CCTCC M 209298 were investigated in flasks. Sodium citrate was found to be beneficial for the high co-production of SAM and GSH. The response surface analysis was applied in the optimization of sodium citrate concentration and addition time, and a strategy of 10 g/L sodium citrate addition at 6 h was predicted by a statistical model and verified to be the best approach for increased co-production of SAM and GSH. Based on the results derived from the kinetic analysis on the batch fermentation processes, intracellular levels of NADH and ATP could be significantly improved by sodium citrate, and which in turn provided essential energy substance needed for the over-production of SAM and GSH. The results also provide a potential approach for efficient production of analogical useful chemicals biosynthesized with the consumption of energy.
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Received: 07 January 2013
Published: 25 August 2013
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