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中国生物工程杂志

China Biotechnology
China Biotechnology  2013, Vol. 33 Issue (6): 111-116    DOI:
    
The Technology Progress of Antibody-producing Cell Line Development
LIU Bo-ning
New Drug Reaserch and Development Center, North China Pharmaceutical Group Corporation and State Key Laboratory of Antibody Drug Reaserch and Development, Shijiazhuang 050015, China
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Abstract  

The commercial therapeutic antibodies manufacturing began with construction of antibody-producing cell line, and the productivity of engineering cell line for antibody expression has reached 20~70pg/cell/day at present. Many industrial cases of various animal cell lines as host cell were summarized, accounting to their different attributes. Furthermore, the latest technology progression of cell line developments was introduced, as follows: Cell engineering technology ensured the host cell with robust growth and high productivity; The New vector element and site-directed integration technology contribute to overcoming the "position effection" by random integration. Lastly, the application of cell-sorting by flow cytometer and automatic screening device have remarkably improved the efficient and throughout of recombinant cell line screening, etc. Additionally, the phenotypic variation and instability of cell line and the technological trend in this field are also discussed.



Key wordsRecombinant antibody      Cell line development      Vector construction      Screening strategy     
Received: 04 February 2013      Published: 25 June 2013
ZTFLH:  Q819  
Cite this article:

LIU Bo-ning. The Technology Progress of Antibody-producing Cell Line Development. China Biotechnology, 2013, 33(6): 111-116.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2013/V33/I6/111

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