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| Medium Optimization for Rhamnolipids Production by Pseudomonas aeruginosa O-2-2 and LC-MS/MS Analysis |
| ZHAO Fang-long, ZHU Ling-qing, YANG Xue, LU Wen-yu |
| Department of Biological Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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Abstract Rhamnolipids are excellent biosurfactants which are widely used in biomedicine, oil recovery and environmental protection. Response surface methodology (RSM) was employed to optimize the medium for rhamnolipids production by Pseudomonas aeruginosa O-2-2. The result of the Plackett-Burman design showed that phosphate, nitrate and trace elements had significant effects on rhamnolipids production. The Box-Behnke design suggested that the optimum values of phosphate, nitrate and trace elements were 3.2g/L, 13.76g/L and 5.17ml, respectively. Rhamnolipids production reached 8.85g/L, which was in agreement with the predicted production of 8.48 g/L. Compared with the production of original level (6.24g/L), 30.8% increment had been obtained. By scaling up the fermentation to 200L fermentor with the optimal medium, the concentration of rhamonlipids reached to 70g/L, and the fermentation period was kept in 110h. When the secondary-distillation was used as a new separation process, the rhamnolipid purity was 86.6%. In general, relative content of each ingredient in this product was: mono-rhamno-mono-lipid 1.79%, mono-rhamno-di-lipid 36.83%, di-rhamno-mono-lipid 39.64%, di-rhamno-di-lipid 20.70%.
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Received: 06 March 2013
Published: 25 June 2013
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