A Preliminary Study of <em>exoD</em> Gene Relating Exopolysaccharides Secretion in <em>Sinorhizobium fredii</em> WGF03

doi:10.13523/j.cb.20140607

China Biotechnology

2014, Vol. 34

Issue (06): 47-54 DOI: 10.13523/j.cb.20140607

A Preliminary Study of exoD Gene Relating Exopolysaccharides Secretion in Sinorhizobium fredii WGF03

MA Ting-ting^1,2, ZHANG Jian^1,2, SU Yue-ping^1,2, SONG Zhang-yang^1,2, TANG Xian-lai⁴, SHEN Pei-hong¹, WU Bo³

1. College of Life Science and Technology of Guangxi University, Nanning 530005, China;
2. The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, Nanning 530005, China;
3. State Key Laboratory for Conservation and Utilization of Agricultural Bioresources in the Subtropics, Nanning 530005, China;
4. The Science and Technology Department of Guangxi, Nanning 530012, China

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Abstract

An extracellular polysaccharide secretion related gene exoD was cloned from genome of S.fredii WGF03 and the influence of genes on extracellular polysaccharide synthesis as well as nodulation,nitrogen fixation with host plant was investigated. ΔexoD mutant was constructed through homologous double-crossover using suicide plasmid pk18mobsacB as a vector. Compared with the wile type strain, the mutant strain produced less exopolysaccharides (EPS) on YMA medium plate and the motility was decreased.Nothing changes in the growth situation on the medium containing less than 350 mmol/L of NaCl. The plant test showed that the number of nodules was more, but small, varying shapes, and nitrogen-fixing enzyme activity was also significantly decreased after inoculation mutant strain. This demonstrated that exoD gene affect EPS synthesis in S.fredii WGF03, and involve in nodulation and nitrogenase activity.

Key words： S. fredii WGF03 EPS Deletion mutation exoD Plant tests

Received: 27 March 2014 Published: 25 June 2014

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Cite this article:

MA Ting-ting, ZHANG Jian, SU Yue-ping, SONG Zhang-yang, TANG Xian-lai, SHEN Pei-hong, WU Bo. A Preliminary Study of exoD Gene Relating Exopolysaccharides Secretion in Sinorhizobium fredii WGF03. China Biotechnology, 2014, 34(06): 47-54.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20140607 OR https://manu60.magtech.com.cn/biotech/Y2014/V34/I06/47

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