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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (2): 62-67    DOI: 10.13523/j.cb.20160209
    
Recombinant Expression of ε-Lysine Acylase from Streptomyces mobaraensis for Synthesis of Nε-lauroyl-L-lysine
CHENG Cai-hong, DU Ting, CHEN Ke-quan, LI Yan
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China
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Abstract  

ε-Lysine acylase from Streptomyces mobaraensis(Sm-ELA) can catalyzes hydrolysis of Nε-lauroyl-L-lysine in water with ε-lysine and lauric acid as the substrates. It's an energy-saving and environmentally friendly way that avoids the high temperature and organic solvent by using the chemical method. Two recombinant plasmids which are pET28a-SmELA and pTrcOmpXK122SmELA were constructed and a normal expression of intracellular and the cell surface were achieved respectively. The recombinant enzymes were used in catalytic synthesis of Nε-lauroyl-L-lysine and the catalytic efficiency was preliminarily compared. Nε-lauroyl-L-lysine was synthesized from 50 mmol/L ε-lysine and 10 mmol/L lauric acid in an aqueous buffer solution at 37℃. The maximum yield was 31. 1% after 24 h of reaction for 10 mmol/L lauric acid.



Key wordsNε-lauroyl-L-lysine      Biocatalysis      Acyl transferase     
Received: 22 July 2015      Published: 22 December 2015
ZTFLH:  Q789  
Cite this article:

CHENG Cai-hong, DU Ting, CHEN Ke-quan, LI Yan. Recombinant Expression of ε-Lysine Acylase from Streptomyces mobaraensis for Synthesis of Nε-lauroyl-L-lysine. China Biotechnology, 2016, 36(2): 62-67.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160209     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I2/62

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