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| Effects of PLGA Degradation on Angiogenesis in vitro |
| WU Yan-ge, SUN Xue-feng, YANG Lin, WANG Zheng |
| Shenzhen People's Hospital, the Second Clinical Medical College of Jinan University, Shenzhen 518020, China |
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Abstract Objective: To investigate the influences of degradable PLGA scaffold on proliferation, migration and the formation of tube-like structure (TLS formation) of vascular endothelial cells.Methods: PLGA scaffold were immersed in PBS fluid to stimulate the degradation for 1, 2, and 4 weeks. HUVEC (Human Umbilical Vein Endothelial Cells) was cultured with degradation fluid. Cell proliferation, migration and TLS formation were detected by Brdu ELISA, Transwell chamber and tube formation.Results: The PLGA degradation fluid showed no effects on the migration, TLS formation of HUVEC, but the proliferation was increased in 1 week. With the prolonged degradation time, the migration and TLS formation were significantly decreased in 2 weeks, and the proliferation, migration and TLS formation were reduced remarkably in 4 weeks.Conclusions: At the beginning of degradation, PLGA could improve the proliferation function. The concentration of acid products increased with the increase of degradation time, and cell behaviors were retrained, thus inhibiting the angiogenesis of endothelial cells.
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Received: 01 February 2012
Published: 25 July 2012
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