|
|
|
| Effects of DKPs on Gene Expression of the Antibacterial Substances in Bacillus amyloliquefaciens Q-426 |
| XIONG Wen1, YANG Xue-min1, WANG Jian-hua3, QUAN Chun-shan1,2, FAN Sheng-di1,2 |
1. College of Life Science, Dalian Nationalities University, Dalian 116600, China;
2. Key Laboratory of State Ethnic Affairs Commission and Ministry of Education, Dalian 116600, China;
3. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China |
|
|
|
Abstract Objective: Bacillus amyloliquefaciens Q-426 could produce varieties of antifungal lipopeptides during its stationary growth process, including bacillomycin D, fengycin A and B. Effects of diketopiperazines (DKPs) as signal molecules of quorum sensing (QS) on the biosynthesis of above antifungal compounds were studied through real time fluorescent quantitative polymerase chain reaction (Real-time Q-PCR). Methods: DKPs at a final concentration of 5 mg/l were added to the culture broth of strain Q-426 which was incubated at 30℃ for 12 h. After continuing cultivation for 48 h, quantitative analysis of mRNA expression levels was carried out by Real-time Q-PCR. Result: DKPs could inhibit the expression of some genes related with the biosynthesis of antifungal lipopeptides.
|
|
Received: 28 November 2011
Published: 25 March 2012
|
|
|
|
|
|
[1] Bott R, Ultsch M, Kossiakoff A, et al. The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8Å and an analysis of the structural consequences of peroxide inactivation. J Biol Chem, 1988, 263(16): 7895-7906.
[2] Chen X H, Koumoutsi A, Scholz R, et al. Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens. J Biotech, 2009, 140(1-2):27-37.
[3] Kozlovsky A, Vinokurova N G, Adanin V M, et al.Piscarinines, new polycyclic diketopiperazine alkaloids from Penicillium piscarium VKM F-691. Nat Prod Lett, 2000, 14 (5): 333-340.
[4] Yoshio H, Sumie O, Koji T, et al. Total synthesis of anti-microtubule diketopiperazine derivatives: phenylahistin and aurantiamine. J Org Chem, 2000, 65(24): 8402-8405.
[5] Holden M T G, Chhabra S R, Denys R, et al. Quorum sensing cross talk: isolation and chemical characterization of cyclic dipeptides from Pseudomonas aeruginosa and other Gram-negative bacteria. Mol Microbiol, 1999, 33(6): 1254-1266.
[6] Degrassi G, Aguilar C, Bosco M, et al. Plant growth-promoting Pseudomonas putida WCS358 produces and secretes four cyclic dipeptides: cross-talk with quorum sensing bacteria sensors. Current Microbiology, 2002, 45(4): 250-254.
[7] Wang J H, Quan C S, Qi X H, et al. Determination of diketopiperazines of Burkholderia cepacia CF-66 by gas chromatography-mass spectrometry. Anal Bioanal Chem, 2010, 396(5): 1773-1779.
[8] Girard B M, May V, Bora S H, et al. Regulation of neurotrophic peptide expression in sympathetic neurons: quantitative analysis using radioimmunoassay and real-time quantitative polymerase chain reaction. Regul Peptides, 2002, 109(1-3): 89-101.
[9] Alexandra G, Gilles F, Christiane C, et al. Tracking T cell clonotypes in complex T lymphocyte populations by real-time quantitative PCR using fluorogenic complementarity-determining region-3-specific probes. Journal of Immunological Methods, 2002, 270(2): 269-280.
[10] Khmel I A, Belik A, Zaitseva Y, et al. Quorum sensing and communication in bacteria. Moscow University Biological Sciences Bulletin, 2008, 63(1): 25-31.
[11] Nealson K H, Hastings J W. Bacterial bioluminescence: its control and ecological significance. Microbiol Rev, 1979, 43(4): 496-518.
[12] Wisniewski-Dyé F, Jones J, Chhabra S R, et al. raiIR genes are part of a quorum-sensing network controlled by CinI and CinR in Rhizobium leguminosarum. J Bacteriol, 2002, 184(6): 1957-1606.
[13] Winson M K, Camara M, Latifi A, et al. Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa. Proc Natl Acad Sci USA, 1995, 92(20): 9427-9431.
[14] Bainton N J, Bycroft B W, Chhabra S R, et al. A general role for the lux autoinducer in bacterial cell signaling control of antibiotic biosynthesis in Erwinia. Gene, 1992, 116(1): 87-91.
[15] Huber B, Riedel K, Hentzer M, et al. The cep quorum-sensing system of Burkholderia cepacia H111 controls biofilm formation and swarming motility. Microbiology, 2001, 147(9): 2517-2528.
[16] Elvers K T, Park S F. Quorum sensing in Campylobacter jejuni: detection of a luxS encoded signaling molecule. Microbiology, 2002, 148(5): 1475-1481.
[17] Fuqua W C, Winans S C. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumour metabolite. J Bacteriol, 1994, 176(10): 2796-2806.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
